Structure of PDB 5j30 Chain QY

Receptor sequence

>5j30QY (length=258) Species: 585035 (Escherichia coli S88)

QQLQVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARR
WRVEIMSASEGEHGGYKEIIAKISGDGVYGRLKFESGGHRVQRVPATESQ
GRIHTSACTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTTDSA
IRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEAST
RRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEP
IIQEHQAD

3D structure

• PDB: 5j30 Uniformity of Peptide Release Is Maintained by Methylation of Release Factors.
• Chain: QY
• Resolution: 3.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	QY	R116 R228 S229 S230 G231 A232 G233 G234 X235 H236 V237 N238 T239 T240 D241 R245 E256 D259 R261 Q263 H264 R295	R23 R135 S136 S137 G138 A139 G140 G141 X142 H143 V144 N145 T146 T147 D148 R152 E163 D166 R168 Q170 H171 R202
BS02	rna	QY	G118 T119 G120 G121 R137 E155 K160 R183 Q193 D302 R303 Q314 R316	G25 T26 G27 G28 R44 E62 K67 R90 Q100 D209 R210 Q221 R223
BS03	rna	QY	G233 G234 X235 E260 R261	G140 G141 X142 E167 R168
BS04	rna	QY	G121 D122 E123 T190 R195 I196 H197 T198	G28 D29 E30 T97 R102 I103 H104 T105
BS05	MG	QY	R116 E161	R23 E68

Gene Ontology

Molecular Function

• GO:0003747 translation release factor activity
• GO:0005515 protein binding
• GO:0016149 translation release factor activity, codon specific
• GO:0043022 ribosome binding

Biological Process

• GO:0006412 translation
• GO:0006415 translational termination

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:5j30, PDBe:5j30, PDBj:5j30
• PDBsum: 5j30
• PubMed: 27681416
• UniProt: P0A7I0|RF1_ECOLI Peptide chain release factor RF1 (Gene Name=prfA)