Structure of PDB 8adl Chain Q

Receptor sequence

>8adlQ (length=782) Species: 4932 (Saccharomyces cerevisiae)

GPYNSPTFGKSLSLKVDGGFNAVSINPSGRDIVLASRQGLYIIDLDDPFT
PPRWLHHITPWQVADVQWSPHPAKPYWIVSTSNQKAIIWNLAKSSSNAIE
FVLHGHSRAITDINFNPQHPDVLATCSVDTYVHAWDMRSPHRPFYSTSSW
RSAASQVKWNYKDPNVLASSHGNDIFVWDLRKGSTPLCSLKGHVSSVNSI
DFNRFKYSEIMSSSNDGTVKFWDYSKSTTESKRTVTTNFPIWRGRYLPFG
EGYCIMPMVGGNNAVYLINLCDDNKKTKLQPIYAFKGHSDRVIDFLWRSR
HTCDGDYDDREFQLVTWSKDCDLKLWPISDSIYGKVNFDRGKRLEEKLPD
YDYCSYNKEPENFRRLRENFVTTSGLKTNHITWLSGIRMNIQNLGEEVSA
IGHKFPKVVFEKISVSTRELCLTLNGPWSEENPDDYIFLRISINFPLNYP
NKGDPPKFTIEENSNLTMSKRQEILSNLATIGQKYTDSNLYCLEPCIRFV
LGETTPVPNGCGSCWTATGELFCFFANKNLVISKNFSSLLSDRKELALEY
LFMDATPEGFARNNALVAEKFDLDEISHCWQILSDMLIDQSDYDPYTTIW
NNHPMGIKWFIKEAIVYFERQQNLQMLAMLCCVILSARRMPDIKVELLHD
DIIEAYEQEDLLHLEVSDIPKFQTYIYQYSKLLFRWGLPLERVKILKVST
DFRSSYSSPYNGVLTHWIENNEFGEEKFLARNCNYCDLRVTRSSFICGNC
QHVLHSSCARIWWEIGDECPSGCGCNCPEMFD

3D structure

• PDB: 8adl Cryo-EM structure of the SEA complex.
• Chain: Q
• Resolution: 2.95 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	Q	C1098 C1101 H1120 C1123	C733 C736 H755 C758
BS02	ZN	Q	C1112 C1115 C1140	C747 C750 C775
BS03	ZN	Q	C1115 H1117 C1134	C750 H752 C769

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0035591 signaling adaptor activity

Biological Process

• GO:0015031 protein transport
• GO:0034198 cellular response to amino acid starvation
• GO:1903432 regulation of TORC1 signaling
• GO:1904263 positive regulation of TORC1 signaling

Cellular Component

• GO:0000329 fungal-type vacuole membrane
• GO:0005773 vacuole
• GO:0005774 vacuolar membrane
• GO:0016020 membrane
• GO:0035859 Seh1-associated complex

External links

• PDB: RCSB:8adl, PDBe:8adl, PDBj:8adl
• PDBsum: 8adl
• PubMed: 36289347
• UniProt: Q03897|WDR59_YEAST Maintenance of telomere capping protein 5 (Gene Name=MTC5)