Structure of PDB 7w59 Chain Q

Receptor sequence

>7w59Q (length=1322) Species: 9606 (Homo sapiens)

NAEFVTQLACKYWAPHIKKKSPFDIKVIEDIYEKEIVKSRFAIRKIMLLE
FSQYLENYLWMNYSPEVSSKAYLMSICCMVNEKFRENVPAWEIFKKKPDH
FPFFFKHILKAALAETDGEFSLHEQTVLLLFLDHCFNSLEVDLIRSQVQQ
LISLPMWMGLQLARLELELKKTPKLRKFWNLIKKNDEKMDPEAREQAYQE
RRFLSQLIQKFISVLKSVPLSEPVTMDKVHYCERFIELMIDLEALLPTRR
WFNTILDDSHLLVHCYLSNLVRREEDGHLFSQLLDMLKFYTGFEINDQTG
NALTENEMTTIHYDRITSLQRAAFAHFPELYDFALSNVAEVDTRESLVKF
FGPLSSNTLHQVASYLCLLPTLPKNEDTTFDKEFLLELLVSRHERRISQI
QQLNQMPLYPTEKIIWDENIVPTEYYSGEGCLALPKLNLQFLTLHDYLLR
NFNLFRLESTYEIRQDIEDSVSRMKPWQSGGVVFGGWARMAQPIVAFTVV
EVAKPNIGENWPTRVRADVTINLNVRDHIKDEWEGLRKHDVCFLITVRPT
KPYGTKFDRRRPFIEQVGLVYVRGCEIQGMLDDKGRVIPRPNLRGESRTF
RVFLDPNQYQQDMTNTIQNGAEDVYETFNIIMRRKPKENNFKAVLETIRN
LMNTDCVVPDWLHDIILGYGDPSSAHYSKMPNQIATLDFNDTFLSIEHLK
ASFPGHNVKVTVEDPALQPFRITFPVEAKTLIVEPHVIPNRGPYPYNQPK
RNTIQFTHTQIEAIRAGMQPGLTMVVGPPGTGKTDVAVQIISNIYHNFPE
QRTLIVTHSNQALNQLFEKIMALDIDERHLLRLGHEELETEKDFSRYGRV
NYVLARRIELLEEVKRLQKSLGVPGDASYTCETAGYFFLYQVMSRWEEYI
SKVKNPDVTEVSTFFPFHEYFANAIFKGRSYEEDMEIAEGCFRHIKKIFT
QLEEFRASELLRSGLDRSKYLLVKEAKIIAMTCTHAALKRHDLVKLGFKY
DNILMEEAAQILEIETFIPLLLQNPQDGFSRLKRWIMIGDHHQLPPVIKN
MAFQKYSNMEQSLFTRFVRVGVPTVDLDAQGRARASLCNLYNWRYKNLGN
LPHVQLLPEFSTANAGLLYDFQLINVEDFQGVGESEPNPYFYQNLGEAEY
VVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCGNNPLIGRPNK
VTTVDRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSRARLGLYIFAR
VSLFQNCFELTPAFSQLTARPLHLHIIPTETTRKNGERPSHEVQIIKNMP
QMANFVYNMYMHLIQTTHHYHQ

3D structure

• PDB: 7w59 Mechanism of exon ligation by human spliceosome.
• Chain: Q
• Resolution: 3.6 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	Q	I800 Q801 F802 G826 G828 T830 G1137	I754 Q755 F756 G780 G782 T784 G1081

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0003727 single-stranded RNA binding
• GO:0003729 mRNA binding
• GO:0004386 helicase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0034458 3'-5' RNA helicase activity

Biological Process

• GO:0000398 mRNA splicing, via spliceosome
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005681 spliceosomal complex
• GO:0016020 membrane
• GO:0071007 U2-type catalytic step 2 spliceosome
• GO:0071013 catalytic step 2 spliceosome

External links

• PDB: RCSB:7w59, PDBe:7w59, PDBj:7w59
• PDBsum: 7w59
• PubMed: 35705093
• UniProt: O60306|AQR_HUMAN RNA helicase aquarius (Gene Name=AQR)