Structure of PDB 5lqw Chain Q

Receptor sequence

>5lqwQ (length=791) Species: 4932 (Saccharomyces cerevisiae)

NVEGIHDLMFFKPSDHKYFADVISKKKERTLSMLLLKIKTASRRTSMRIL
TDKAVTFGPEMIFNRLLPILLDRSLEDQERHLMIKTIDRVLYQLGDLTKP
YVHKILVVAAPLLIDEDPMVRSTGQEIITNLSTVAGLKTILTVMRPDIEN
EDEYVRNVTSRAAAVVAKALGVNQLLPFINAACHSRKSWKARHTGIKIVQ
QIGILLGIGVLNHLTGLMSCIKDCLMDHVPVRIVTAHTLSTLAENSYPYG
IEVFNVVLEPLWKGIRSHVLSSFLKAVGSMIPLMDPEYAGYYTTEAMRII
RREFDSDEMKKTILLVLQKCSAVESITPKFLREEIAPEFFQKFWVRRVAL
DRPLNKVVTYTTVTLAKKLGSYTIDKLLTPLRDEAEPFRTMAVHAVTRTV
NLLGTADLDERLETRLIDALLIAFQEQTNSDSIIFKGFGAVTVSLDIRMK
PFLAPIVSTILNHLKHKTPLVRQHAADLCAILIPVIKNCHEFEMLNKLNI
ILYESLGEVYPEVLGSIINAMYCITSVMDLDPINQILPTLTPILRNKHRK
VEVNTIKFVGLIGKLPKEWMRICFELLELLKSTNKEIRRSANATFGFIAP
HDVLVALLNNRVCTAVAIGIVAKVCGPYNVLPVIMNEYTTPETNVQNGVL
KAMSFMFEYIGNMSKDYIYFITPLLEDALTDLVHRQTASNVITHLALNCS
GTGHEDAFIHLMNLLIPNIFETSPHAIMRILEGLEALSQALGPGLFMNYI
WAGLFHPAKNVRKAFWRVYNNMYVMYQDAMVPFYPVTPDNN

3D structure

• PDB: 5lqw Molecular architecture of the Saccharomyces cerevisiae activated spliceosome
• Chain: Q
• Resolution: 5.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	Q	A179 S892 A927 K928 N929	A41 S723 A758 K759 N760
BS02	rna	Q	V852 H894	V683 H725

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0005515 protein binding

Biological Process

• GO:0000245 spliceosomal complex assembly
• GO:0000398 mRNA splicing, via spliceosome
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing
• GO:1903241 U2-type prespliceosome assembly

Cellular Component

• GO:0005634 nucleus
• GO:0005681 spliceosomal complex
• GO:0005684 U2-type spliceosomal complex
• GO:0005686 U2 snRNP
• GO:0071004 U2-type prespliceosome
• GO:0071013 catalytic step 2 spliceosome

External links

• PDB: RCSB:5lqw, PDBe:5lqw, PDBj:5lqw
• PDBsum: 5lqw
• PubMed: 27562955
• UniProt: P49955|SF3B1_YEAST U2 snRNP component HSH155 (Gene Name=HSH155)