Structure of PDB 5hyn Chain Q
Receptor sequence
>5hynQ (length=565) Species:
9606
(Homo sapiens) [
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PVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWK
QRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMY
SWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDR
ECGFINDEIFVELVNALGQPSDKIFEAISSMFPDKGTAEELKEKYKELTE
CTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRK
NTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTEPPENVEWSGAEAS
MFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIANHVYNYQPCD
HPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCY
LAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGW
GIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFV
VDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFF
DYRYSQADALKYVGI
3D structure
PDB
5hyn
Structural basis of oncogenic histone H3K27M inhibition of human polycomb repressive complex 2.
Chain
Q
Resolution
2.95 Å
3D
structure
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Enzymatic activity
Enzyme Commision number
2.1.1.356
: [histone H3]-lysine(27) N-trimethyltransferase.
Interaction with ligand
Site
#
Ligand
Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01
peptide
Q
Y641 Q648 D652 G655 L666 F667 F724 Y726 R727
Y467 Q474 D478 G481 L492 F493 F550 Y552 R553
BS02
ZN
Q
C286 C289 C294 H297
C227 C230 C235 H238
BS03
ZN
Q
C320 C463
C261 C325
BS04
ZN
Q
C530 C543 C549 C553
C356 C369 C375 C379
BS05
ZN
Q
C523 H525 C530 C534
C349 H351 C356 C360
BS06
ZN
Q
C523 C543 C547
C349 C369 C373
BS07
ZN
Q
C566 C580 C588 C601
C392 C406 C414 C427
BS08
ZN
Q
C560 C573 C580
C386 C399 C406
BS09
ZN
Q
C560 C562 C566 C571
C386 C388 C392 C397
BS10
SAH
Q
V621 A622 W624 M662 C663 S664 F665 N688 H689 Y726 A733 L734 K735
V447 A448 W450 M488 C489 S490 F491 N514 H515 Y552 A559 L560 K561
BindingDB: IC50=467nM
Gene Ontology
Molecular Function
GO:0000976
transcription cis-regulatory region binding
GO:0000978
RNA polymerase II cis-regulatory region sequence-specific DNA binding
GO:0000979
RNA polymerase II core promoter sequence-specific DNA binding
GO:0001222
transcription corepressor binding
GO:0003677
DNA binding
GO:0003682
chromatin binding
GO:0003714
transcription corepressor activity
GO:0003723
RNA binding
GO:0005515
protein binding
GO:0008168
methyltransferase activity
GO:0016279
protein-lysine N-methyltransferase activity
GO:0031490
chromatin DNA binding
GO:0031491
nucleosome binding
GO:0042054
histone methyltransferase activity
GO:0042393
histone binding
GO:0043021
ribonucleoprotein complex binding
GO:0043565
sequence-specific DNA binding
GO:0046976
histone H3K27 methyltransferase activity
GO:0070878
primary miRNA binding
GO:0106222
lncRNA binding
GO:0140938
histone H3 methyltransferase activity
GO:0140951
histone H3K27 trimethyltransferase activity
GO:1990841
promoter-specific chromatin binding
Biological Process
GO:0000082
G1/S transition of mitotic cell cycle
GO:0000122
negative regulation of transcription by RNA polymerase II
GO:0006325
chromatin organization
GO:0006338
chromatin remodeling
GO:0006346
DNA methylation-dependent heterochromatin formation
GO:0006355
regulation of DNA-templated transcription
GO:0006357
regulation of transcription by RNA polymerase II
GO:0008284
positive regulation of cell population proliferation
GO:0009913
epidermal cell differentiation
GO:0010468
regulation of gene expression
GO:0010629
negative regulation of gene expression
GO:0010718
positive regulation of epithelial to mesenchymal transition
GO:0014013
regulation of gliogenesis
GO:0014834
skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration
GO:0014898
cardiac muscle hypertrophy in response to stress
GO:0021695
cerebellar cortex development
GO:0021766
hippocampus development
GO:0030097
hemopoiesis
GO:0030183
B cell differentiation
GO:0030216
keratinocyte differentiation
GO:0030335
positive regulation of cell migration
GO:0031048
regulatory ncRNA-mediated heterochromatin formation
GO:0031507
heterochromatin formation
GO:0031509
subtelomeric heterochromatin formation
GO:0032259
methylation
GO:0032355
response to estradiol
GO:0034244
negative regulation of transcription elongation by RNA polymerase II
GO:0035984
cellular response to trichostatin A
GO:0036211
protein modification process
GO:0036333
hepatocyte homeostasis
GO:0042127
regulation of cell population proliferation
GO:0042752
regulation of circadian rhythm
GO:0043406
positive regulation of MAP kinase activity
GO:0043547
positive regulation of GTPase activity
GO:0045605
negative regulation of epidermal cell differentiation
GO:0045617
negative regulation of keratinocyte differentiation
GO:0045814
negative regulation of gene expression, epigenetic
GO:0045892
negative regulation of DNA-templated transcription
GO:0048387
negative regulation of retinoic acid receptor signaling pathway
GO:0048511
rhythmic process
GO:0048863
stem cell differentiation
GO:0050767
regulation of neurogenesis
GO:0051154
negative regulation of striated muscle cell differentiation
GO:0051932
synaptic transmission, GABAergic
GO:0070301
cellular response to hydrogen peroxide
GO:0070314
G1 to G0 transition
GO:0071168
protein localization to chromatin
GO:0071902
positive regulation of protein serine/threonine kinase activity
GO:0090183
regulation of kidney development
GO:0097421
liver regeneration
GO:0140718
facultative heterochromatin formation
GO:1900006
positive regulation of dendrite development
GO:1900016
negative regulation of cytokine production involved in inflammatory response
GO:1902808
positive regulation of cell cycle G1/S phase transition
GO:1904772
response to tetrachloromethane
GO:2000134
negative regulation of G1/S transition of mitotic cell cycle
GO:2000737
negative regulation of stem cell differentiation
Cellular Component
GO:0000781
chromosome, telomeric region
GO:0000785
chromatin
GO:0005634
nucleus
GO:0005654
nucleoplasm
GO:0005677
chromatin silencing complex
GO:0005694
chromosome
GO:0005721
pericentric heterochromatin
GO:0035098
ESC/E(Z) complex
GO:0045120
pronucleus
GO:0045202
synapse
View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB
RCSB:5hyn
,
PDBe:5hyn
,
PDBj:5hyn
PDBsum
5hyn
PubMed
27121947
UniProt
Q15910
|EZH2_HUMAN Histone-lysine N-methyltransferase EZH2 (Gene Name=EZH2)
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