Structure of PDB 5ady Chain Q

Receptor sequence

>5adyQ (length=117) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRR
QRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVF
DKVAFTALVEKAKAALA

3D structure

PDB

5ady Hflx is a Ribosome Splitting Factor Rescuing Stalled Ribosomes Under Stress Conditions

Chain

Resolution

4.5 Å

3D
structure

Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	Q	A1 R2 V3 K4 R5 R10 R12 K21 G22 Y23 Y24 G25 A26 R27 R29 V30 Y31 R32 V33 Q36 K40 Y44 Y46 D48 R50 Q51 R52 R54 Q55 F56 R57 Q58 W60 N65 R69 S74 Y75 S76 I79 N80 K83 R91 K92	A1 R2 V3 K4 R5 R10 R12 K21 G22 Y23 Y24 G25 A26 R27 R29 V30 Y31 R32 V33 Q36 K40 Y44 Y46 D48 R50 Q51 R52 R54 Q55 F56 R57 Q58 W60 N65 R69 S74 Y75 S76 I79 N80 K83 R91 K92

Gene Ontology

	Molecular Function
GO:0000900	mRNA regulatory element binding translation repressor activity
GO:0003729	mRNA binding
GO:0003735	structural constituent of ribosome
GO:0019843	rRNA binding
GO:0070180	large ribosomal subunit rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation
GO:0017148	negative regulation of translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

External links

PDB	RCSB:5ady, PDBe:5ady, PDBj:5ady
PDBsum	5ady
PubMed	26458047
UniProt	P0A7L3\|RL20_ECOLI Large ribosomal subunit protein bL20 (Gene Name=rplT)