Structure of PDB 7s38 Chain P

Receptor sequence
>7s38P (length=1353) Species: 301447 (Streptococcus pyogenes serotype M1) [Search protein sequence]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILL
SDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFF
DQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQ
RTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYV
GPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNL
PNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL
FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIK
DKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLK
RRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMG
RHKPENIVIEMAQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKL
YLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSD
KNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSEL
DKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSK
LVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYG
DYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRP
LIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVK
ELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRK
RMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQ
HKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIH
LFTLTNLGAPAAFKYFDTTIDRKRYCSTKEVLDATLIHQSITGLYETRID
LSQ
3D structure
PDB7s38 CRISPR-Cas9 bends and twists DNA to read its sequence.
ChainP
Resolution3.3 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna P T249 K377 K1107 R1114 S1116 D1135 S1136 K1200 A1215 S1216 Q1221 A1320 R1333 R1335 T247 K375 K1096 R1103 S1105 D1124 S1125 K1189 A1204 S1205 Q1210 A1309 R1322 R1324
BS02 rna P S29 K30 K31 L35 I43 K44 N46 G56 E57 T58 A59 T62 R63 R66 T67 R69 R70 R71 Y72 R74 R75 N77 R78 Y81 L101 S104 F105 R115 H116 I122 E125 V126 H129 T134 I135 H160 K163 F164 R165 G166 Y325 H328 H329 L332 R340 F351 Y359 I363 Q402 R403 T404 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 R467 T472 I473 P475 M495 K500 R661 S730 K735 K742 K749 R753 M1089 Q1091 K1097 E1099 V1100 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 Y1131 F1134 M1169 E1225 A1227 Q1272 H1349 Q1350 S1351 Y1356 E1357 S27 K28 K29 L33 I41 K42 N44 G54 E55 T56 A57 T60 R61 R64 T65 R67 R68 R69 Y70 R72 R73 N75 R76 Y79 L99 S102 F103 R113 H114 I120 E123 V124 H127 T132 I133 H158 K161 F162 R163 G164 Y323 H326 H327 L330 R338 F349 Y357 I361 Q400 R401 T402 N405 G406 F444 R445 I446 Y448 G451 P452 L453 A454 R455 N457 S458 F460 R465 T470 I471 P473 M493 K498 R659 S728 K733 K740 K747 R751 M1078 Q1080 K1086 E1088 V1089 T1091 G1092 G1093 F1094 I1099 P1101 K1102 R1111 K1112 K1113 Y1120 F1123 M1158 E1214 A1216 Q1261 H1338 Q1339 S1340 Y1345 E1346
BS03 dna P E223 R778 K1107 E1108 S1109 K1334 R1335 C1337 S1338 K1340 E221 R767 K1096 E1097 S1098 K1323 R1324 C1326 S1327 K1329
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:7s38, PDBe:7s38, PDBj:7s38
PDBsum7s38
PubMed35422516
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

[Back to BioLiP]