Structure of PDB 4s2t Chain P

Receptor sequence

>4s2tP (length=615) Species: 6239 (Caenorhabditis elegans)

HMTALEKLAKLRSLFHSERVLALTSSKPMVAYLLPSTDAHHSEYLADYDF
RVKFLSGFSGSNAYVVVTDREALLWTDGRYFTQAGNQLDSNSWKLMKQGQ
PDSITVVDWLVRELERGSVIGFDPTLSTFDAGSKTFKRLKAAGLQPVSIP
GNLVDEFWTDRPRLAGEPVVVLDVEDTGLTTSKKVENLREKLKQKKCDAA
VFTLLDDVMWLLNIRGSDIPYNPLAYSYLFVAMREIHVFIDNEKLDEKSR
AHFHKSNVSIHPYGEVYSWISNWLKAKEASKEPHMVYLTPETNYAIGSII
GEENSMVDTSLVQTAKATKNDHEMQGMRNSHLRDSAALVEFLCWLEKELL
SGKRYTEIELADKIDHLRSLQDKYVTLSFDTISAVGDHAALPHYKPLGES
GNRKAAANQVFLLDSGAHYGDGTTDVTRTVWYTNPPKEFILHNTLVLKGH
INLARAKFPDGIYGSRLDTLTRDALWKLGLDFEHGTGHGVGHYLNVHEGP
IGIGHRPTGGELHASQVLTIEPGFYAKEKYGIRIENCYETVEAVVMSKAQ
NFLTFKSLTLVPIQTSIVDKSLLIEEEINWLNQYHARVLKEVGEHLQKRG
KTDELKWLAEACKPI

3D structure

• PDB: 4s2t Crystal structure of X-prolyl aminopeptidase from Caenorhabditis elegans: A cytosolic enzyme with a di-nuclear active site.
• Chain: P
• Resolution: 2.15 Å

Enzymatic activity

• Enzyme Commision number: 3.4.11.9: Xaa-Pro aminopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	P	Y43 R78 F378 I381 H392 K394 D424 H483 G486 H487 H496 E522 E536	Y44 R79 F379 I382 H393 K395 D425 H484 G487 H488 H497 E521 E535
BS02	ZN	P	D413 D424 E536	D414 D425 E535
BS03	ZN	P	D424 H487 E522 E536	D425 H488 E521 E535

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0008270 zinc ion binding
• GO:0042803 protein homodimerization activity
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0051603 proteolysis involved in protein catabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4s2t, PDBe:4s2t, PDBj:4s2t
• PDBsum: 4s2t
• PubMed: 25905034
• UniProt: O44750|XPP_CAEEL Xaa-Pro aminopeptidase app-1 (Gene Name=app-1)