Structure of PDB 8p4f Chain O

Receptor sequence

>8p4fO (length=695) Species: 9606 (Homo sapiens)

VDWRDEPEPSACEQVSWFPECTTEIPDTQEMSDWMVVGKRKMIIEDETEF
CGEELLHSVLQCKSVFDVLDGEEMRRARTRANPYEMIRGVFFLNRAAMKM
ANMDFVFDRMFTNPRDSYGKPLVKDREAELLYFADVCAGPGGFSEYVLWR
KKWHAKGFGMTLKGPNDFKLEDFYSASSELFEPYYGEGGIDGDGDITRPE
NISAFRNFVLDNTDRKGVHFLMADGGFSVEGQENLQEILSKQLLLCQFLM
ALSIVRTGGHFICKTFDLFTPFSVGLVYLLYCCFERVCLFKPITSRPANS
ERYVVCKGLKVGIDDVRDYLFAVNIKLNQLRNTDSDVNLVVPLEVIKGDH
EFTDYMIRSNESHCSLQIKALAKIHAFVQDTTLSEPRQAEIRKECLRLWG
IPDQARVAPSSSDPKSKFFELIQGTEIDIFSYKPTLLTSKTLEKIRPVFD
YRCMVSGSEQKFLIGLGKSQIYTWDGRQSDRWIKLDLKTELPRDTLLSVE
IVHELKGEGKAQRKISAIHILDVLVLNGTDVREQHFNQRIQLAEKFVKAV
SKPSRPDMNPIRVKEVYRLEEMEKIFVRLEMKIIKGSSGTPKLSYTGRDD
RHFVPMGLYIVRTVNEPWTMGFSKSFKKKFFYNKKTKDSTFDLPADSIAP
FHICYYGRLFWEWGDGIRVHDSQKPQDQDKLSKEDVLSFIQMHRA

3D structure

• PDB: 8p4f Structural insights into human co-transcriptional capping.
• Chain: O
• Resolution: 4.0 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.57: methyltransferase cap1.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	O	N234 R235 K239 S368 K404 E441	N94 R95 K99 S228 K264 E301
BS02	MGT	O	K203 S204 D207 M214 R218 E373 A438 N439	K63 S64 D67 M74 R78 E233 A298 N299
BS03	SAM	O	A236 C277 A278 G279 P280 G281 G282 F283 L302 N306 G334 I336 D364 G366 L383	A96 C137 A138 G139 P140 G141 G142 F143 L162 N166 G194 I196 D224 G226 L243

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
• GO:0008168 methyltransferase activity

Biological Process

• GO:0006370 7-methylguanosine mRNA capping
• GO:0006397 mRNA processing
• GO:0032259 methylation

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:8p4f, PDBe:8p4f, PDBj:8p4f
• PDBsum: 8p4f
• PubMed: 37369200
• UniProt: Q8N1G2|CMTR1_HUMAN Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (Gene Name=CMTR1)