Structure of PDB 8p4e Chain O

Receptor sequence

>8p4eO (length=695) Species: 9606 (Homo sapiens)

VDWRDEPEPSACEQVSWFPECTTEIPDTQEMSDWMVVGKRKMIIEDETEF
CGEELLHSVLQCKSVFDVLDGEEMRRARTRANPYEMIRGVFFLNRAAMKM
ANMDFVFDRMFTNPRDSYGKPLVKDREAELLYFADVCAGPGGFSEYVLWR
KKWHAKGFGMTLKGPNDFKLEDFYSASSELFEPYYGEGGIDGDGDITRPE
NISAFRNFVLDNTDRKGVHFLMADGGFSVEGQENLQEILSKQLLLCQFLM
ALSIVRTGGHFICKTFDLFTPFSVGLVYLLYCCFERVCLFKPITSRPANS
ERYVVCKGLKVGIDDVRDYLFAVNIKLNQLRNTDSDVNLVVPLEVIKGDH
EFTDYMIRSNESHCSLQIKALAKIHAFVQDTTLSEPRQAEIRKECLRLWG
IPDQARVAPSSSDPKSKFFELIQGTEIDIFSYKPTLLTSKTLEKIRPVFD
YRCMVSGSEQKFLIGLGKSQIYTWDGRQSDRWIKLDLKTELPRDTLLSVE
IVHELKGEGKAQRKISAIHILDVLVLNGTDVREQHFNQRIQLAEKFVKAV
SKPSRPDMNPIRVKEVYRLEEMEKIFVRLEMKIIKGSSGTPKLSYTGRDD
RHFVPMGLYIVRTVNEPWTMGFSKSFKKKFFYNKKTKDSTFDLPADSIAP
FHICYYGRLFWEWGDGIRVHDSQKPQDQDKLSKEDVLSFIQMHRA

3D structure

• PDB: 8p4e Structural insights into human co-transcriptional capping.
• Chain: O
• Resolution: 3.9 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.57: methyltransferase cap1.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	O	K203 S204 D207 M214 R218 E373 A438 N439	K63 S64 D67 M74 R78 E233 A298 N299

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
• GO:0008168 methyltransferase activity

Biological Process

• GO:0006370 7-methylguanosine mRNA capping
• GO:0006397 mRNA processing
• GO:0032259 methylation

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:8p4e, PDBe:8p4e, PDBj:8p4e
• PDBsum: 8p4e
• PubMed: 37369200
• UniProt: Q8N1G2|CMTR1_HUMAN Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (Gene Name=CMTR1)