Structure of PDB 8bap Chain O

Receptor sequence

>8bapO (length=525) Species: 101510 (Rhodococcus jostii RHA1)

TRTLPPGVSDERFDAALQRFRDVVGDKWVLSTADELEAFRDPYPVGAAEA
NLPSAVVSPESTEQVQDIVRIANEYGIPLHPVSTGKNNGYGGAAPRLSGS
VIVKTGERMNRILEVNEKYGYALLEPGVTYFDLYEYLQSHDSGLMLDCPD
LGWGSVVGNTLDRGVGYTPYGDHFMWQTGLEVVLPQGEVMRTGMGALPGS
DAWQLFPYGFGPFPDGMFTQSNLGIVTKMGIALMQRPPASQSFLITFDKE
EDLEQIVDIMLPLRINMAPLQNVPVLRNIFMDAAAVSKRTEWFDGDGPMP
AEAIERMKKDLDLGFWNFYGTLYGPPPLIEMYYGMIKEAFGKIPGARFFT
HEERDDRGGHVLQDRHKINNGIPSLDELQQLDWVPNGGHIGFVPVSAPDG
REAMKQFEMVRNRANEYNKDYMAQFVIGLREMYHVCLFIYDTADPEAREE
ILQMTKVLVREAAEAGYGEYRTHNALMDDVMATFNWGDGALLKFHEKIKD
ALDPNGIIAPGKSGIWPQRFRGQNL

3D structure

• PDB: 8bap One-pot biocatalytic synthesis of rac-syringaresinol from a lignin-derived phenol
• Chain: O
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 1.1.3.38: vanillyl-alcohol oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	O	P82 V83 S84 T85 G86 K87 N88 N89 P150 D151 L152 G155 S156 G159 N160 G165 V166 Y168 V227 H390 R472 K513	P81 V82 S83 T84 G85 K86 N87 N88 P149 D150 L151 G154 S155 G158 N159 G164 V165 Y167 V226 H389 R471 K512
BS02	55B	O	Y91 D151 V166 Y168 M282 V436 L438 Y471	Y90 D150 V165 Y167 M281 V435 L437 Y470
BS03	CA	O	D400 R402	D399 R401

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004458 D-lactate dehydrogenase (cytochrome) activity
• GO:0008720 D-lactate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0018465 vanillyl-alcohol oxidase activity
• GO:0046872 metal ion binding
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:1903457 lactate catabolic process

External links

• PDB: RCSB:8bap, PDBe:8bap, PDBj:8bap
• PDBsum: 8bap
• PubMed: 38026814
• UniProt: Q0SBK1