Structure of PDB 6rie Chain O

Receptor sequence
>6rieO (length=822) Species: 10245 (Vaccinia virus) [Search protein sequence]
MDANVVSFSTIATYIDALAKNASELEQRSTAYEINNELELVFIKPPLITL
TNVVNISTIQESFIRFTVTNKEGVKIRTKIPLSKVHGLDVKNVQLVDAID
NIVWEKKSLVTENRLHKECLLRLSTEERHIFLDYKKYGSSIRLELVNLIQ
AKTKNFTIDFKLKYFLGSGAQSKSSLLHAINHPKSRPNTSLEIEFTPRDN
ETVPYDELIKELTTLSRHIFMASPENVILSPPINAPIKTFMLPKQDIVGL
DLENLYAVTKTDGIPITIRVTSNGLYCYFTHLGYIIRYPVKRIIDSEVVV
FGEAVKDKNWTVYLIKLIENDRLEESKYVESKLVDICDRIVFKSKKYEGP
FTTTSEVVDMLSTYLPKQPEGVILFYSKGPKSNIDFKIKKENTIDQTANV
VFRYMSSEPIIFGESSIFVEYKKFSNDKGFPKEYGSGKIVLYNGVNYLNN
IYCLEYINTHNEVGIKSVVVPIKFIAEFLVNGEILKPRIDKTMKYINSED
YYGNQHNIIVEHLRDQSIKIGDINDKFRLNPEVSYFTNKRTRGPLGILSN
YVKTLLISMYCSKTFLDDSNKRKVLAIDFGNGADLEKYFYGEIALLVATD
PDADAIARGNERYNKLNSKTKYYKFDYIQETIRSDTFVSSVREVFYFGKF
NIIDWQFAIHYSFHPRHYATVMNNLSELTASGGKVLITTMDGDKLSKLTD
KKTFIIHKNLPSSENYMSVEKIADDRIVVYNPSTMSTPMTEYIIKKNDIV
RVFNEYGFVLVDNVDFATIIERSKKFINGASTMEDRPSTKNFFELNRGAI
KCEGLDVEDLLSYYVVYVFSKR
3D structure
PDB6rie Structural Basis of Poxvirus Transcription: Transcribing and Capping Vaccinia Complexes.
ChainO
Resolution3.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
2.7.7.50: mRNA guanylyltransferase.
3.6.1.74: mRNA 5'-phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna O E39 I43 K75 R77 K107 R122 R142 K161 F165 E192 E39 I43 K75 R77 K107 R122 R142 K161 F165 E192
BS02 SAM O R548 L549 Y555 F556 D598 F599 D604 D620 R655 Q678 A680 Y683 R528 L529 Y535 F536 D578 F579 D584 D600 R633 Q656 A658 Y661
BS03 MG O E39 E194 E39 E194
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0004482 mRNA 5'-cap (guanine-N7-)-methyltransferase activity
GO:0004484 mRNA guanylyltransferase activity
GO:0004651 polynucleotide 5'-phosphatase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0008168 methyltransferase activity
GO:0016779 nucleotidyltransferase activity
GO:0016787 hydrolase activity
GO:0046872 metal ion binding
GO:0050355 inorganic triphosphate phosphatase activity
GO:0140818 mRNA 5'-triphosphate monophosphatase activity
Biological Process
GO:0006353 DNA-templated transcription termination
GO:0006370 7-methylguanosine mRNA capping
GO:0032259 methylation
GO:0106005 RNA 5'-cap (guanine-N7)-methylation
Cellular Component
GO:0044423 virion component

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6rie, PDBe:6rie, PDBj:6rie
PDBsum6rie
PubMed31835031
UniProtP04298|MCEL_VACCW mRNA-capping enzyme catalytic subunit (Gene Name=OPG113)

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