Structure of PDB 6ouo Chain O

Receptor sequence

>6ouoO (length=116) Species: 562 (Escherichia coli)

DKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAA
STVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHG
RVQALADAAREAGLQF

3D structure

• PDB: 6ouo The structural basis for release-factor activation during translation termination revealed by time-resolved cryogenic electron microscopy.
• Chain: O
• Resolution: 3.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	O	R9 R10 R13 K17 L18 S91 R94 Q98 Y99 R111 F117	R8 R9 R12 K16 L17 S90 R93 Q97 Y98 R110 F116
BS02	rna	O	K3 K4 R15 R25 H29 R30 T31 R33 H34 Y36 Q38 I40 G44 S45 E46 V47 A50 V54 E55 K56 K63 Y64 N67 K68 H100 G101 R102	K2 K3 R14 R24 H28 R29 T30 R32 H33 Y35 Q37 I39 G43 S44 E45 V46 A49 V53 E54 K55 K62 Y63 N66 K67 H99 G100 R101

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6ouo, PDBe:6ouo, PDBj:6ouo
• PDBsum: 6ouo
• PubMed: 31189921
• UniProt: P0C018|RL18_ECOLI Large ribosomal subunit protein uL18 (Gene Name=rplR)