Structure of PDB 4v4d Chain O

Receptor sequence

>4v4dO (length=875) Species: 35816 (Pelobacter acidigallici)

MGEVVRLTNSSTGGPVFVYVKDGKIIRMTPMDFDDAVDAPSWKIEARGKT
FTPPRKTSIAPYTAGFKSMIYSDLRIPYPMKRKSFDPNGERNPQLRGAGL
SKQDPWSDYERISWDEATDIVVAEINRIKHAYGPSAILSTPSSHHMWGNV
GYRHSTYFRFMNMMGFTYADHNPDSWEGWHWGGMHMWGFSWRLGNPEQYD
LLEDGLKHAEMIVFWSSDPETNSGIYAGFESNIRRQWLKDLGVDFVFIDP
HMNHTARLVADKWFSPKIGTDHALSFAIAYTWLKEDSYDKEYVAANAHGF
EEWADYVLGKTDGTPKTCEWAEEESGVPACEIRALARQWAKKNTYLAAGG
LGGWGGACRASHGIEWARGMIALATMQGMGKPGSNMWSTTQGVPLDYEFY
FPGYAEGGISGDCENSAAGFKFAWRMFDGKTTFPSPSNLNTSAGQHIPRL
KIPECIMGGKFQWSGKGFAGGDISHQLHQYEYPAPGYSKIKMFWKYGGPH
LGTMTATNRYAKMYTHDSLEFVVSQSIWFEGEVPFADIILPACTNFERWD
ISEFANCSGYIPDNYQLCNHRVISLQAKCIEPVGESMSDYEIYRLFAKKL
NIEEMFSEGKDELAWCEQYFNATDMPKYMTWDEFFKKGYFVVPDNPNRKK
TVALRWFAEGREKDTPDWGPRLNNQVCRKGLQTTTGKVEFIATSLKNFEE
QGYIDEHRPSMHTYVPAWESQKHSPLAVKYPLGMLSPHPRFSMHTMGDGK
NSYMNYIKDHRVEVDGYKYWIMRVNSIDAEARGIKNGDLIRAYNDRGSVI
LAAQVTECLQPGTVHSYESCAVYDPLGTAGKSADRGGCINILTPDRYISK
YACGMANNTALVEIEKWDGDKYEIY

3D structure

• PDB: 4v4d Crystal structure of pyrogallol-phloroglucinol transhydroxylase, an Mo enzyme capable of intermolecular hydroxyl transfer between phenols
• Chain: O
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y62 S142 H144 D174 W176 E177 D396 Y404
• Catalytic site (residue number reindexed from 1): Y62 S142 H144 D174 W176 E177 D396 Y404
• Enzyme Commision number: 1.97.1.2: pyrogallol hydroxytransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	O	W339 A340 K342 Q377	W339 A340 K342 Q377
BS02	MGD	O	S142 S143 H144 H145 M146 D174 S175 R359 Y496 P499 T503 Q525 I527 D589 S736 H738 H744 T745 M746 E818 N840 M855 N857 N858	S142 S143 H144 H145 M146 D174 S175 R359 Y496 P499 T503 Q525 I527 D589 S736 H738 H744 T745 M746 E818 N840 M855 N857 N858
BS03	MGD	O	Y62 H144 S175 W215 S216 T221 N222 G224 I225 D249 P250 D271 G350 W354 G355 G356 A357 R359 H362 L735 P737 H738 P739 S742 H744	Y62 H144 S175 W215 S216 T221 N222 G224 I225 D249 P250 D271 G350 W354 G355 G356 A357 R359 H362 L735 P737 H738 P739 S742 H744
BS04	PYG	O	S143 H144 D174 S175 W176 Y404 F468	S143 H144 D174 S175 W176 Y404 F468

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0018706 pyrogallol hydroxytransferase activity
• GO:0030151 molybdenum ion binding
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding

Biological Process

• GO:0009061 anaerobic respiration

Cellular Component

• GO:0030288 outer membrane-bounded periplasmic space

External links

• PDB: RCSB:4v4d, PDBe:4v4d, PDBj:4v4d
• PDBsum: 4v4d
• PubMed: 15284442
• UniProt: P80563|PGTL_PELAC Pyrogallol hydroxytransferase large subunit (Gene Name=athL)