Structure of PDB 3h1c Chain O

Receptor sequence

>3h1cO (length=544) Species: 562 (Escherichia coli)

MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTVVGQKKA
KPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIR
PLFPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIG
AARVGYINDQYVLNPTQDELKESKLDLVVAGTEAAVLMVESEAQLLSEDQ
MLGAVVFGHEQQQVVIQNINELVKEAGKPRWDWQPEPVNEALNARVAALA
EARLSDAYRITDKQERYAQVDVIKSETIATLLAEDETLDENELGEILHAI
EKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVLPRTHGSALFTRGETQA
LVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGMVGSPKRRE
IGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRD
GISALQMDIKIEGITKEIMQVALNQAKGARLHILGVMEQAINAP

3D structure

• PDB: 3h1c Crystal structure of Escherichia coli polynucleotide phosphorylase core bound to RNase E, RNA and manganese: implications for catalytic mechanism and RNA degradosome assembly
• Chain: O
• Resolution: 3.57 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.8: polyribonucleotide nucleotidyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	O	E19 M22	E19 M22
BS02	peptide	O	D322 M323 I324 G326 L327 D328 V329 R330 T331 G332 V333 P335 G528 A540 I541	D322 M323 I324 G326 L327 D328 V329 R330 T331 G332 V333 P335 G528 A540 I541
BS03	WO4	O	H403 G436 S438	H403 G436 S438

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004654 polyribonucleotide nucleotidyltransferase activity

Biological Process

• GO:0006396 RNA processing
• GO:0006402 mRNA catabolic process

External links

• PDB: RCSB:3h1c, PDBe:3h1c, PDBj:3h1c
• PDBsum: 3h1c
• PubMed: 19327365
• UniProt: P05055|PNP_ECOLI Polyribonucleotide nucleotidyltransferase (Gene Name=pnp)