Structure of PDB 1xup Chain O

Receptor sequence

>1xupO (length=487) Species: 37734 (Enterococcus casseliflavus)

YVMAIDQGTTSSRAIIFDRNGKKIGSSQKEFPQYFPKSGWVEHNANEIWN
SVQSVIAGAFIESGIRPEAIAGIGITNQRETTVVWDKTTGQPIANAIVWQ
SRQSSPIADQLKVDGHTEMIHEKTGLVIDAYFSATKVRWLLDNIEGAQEK
ADNGELLFGTIDSWLVWKLTDGQVHVTDYSNASRTMLYNIHKLEWDQEIL
DLLNIPSSMLPEVKSNSEVYGHTRSYHFYGSEVPIAGMAGDQQAALFGQM
AFEKGMIKNTYGTGAFIVMNTGEEPQLSDNDLLTTIGYGINGKVYYALEG
SIFVAGSAIQWLRDGLRMIETSPQSEELAAKAKGDNEVYVVPAFTGLGAP
YWDSEARGAVFGLTRGTTKEDFVRATLQAVAYQSKDVIDTMKKDSGIDIP
LLKVDGGAAKNDLLMQFQADILDIDVQRAANLETTALGAAYLAGLAVGFW
KDLDELKSMAEEGQMFTPEMPAEERDNLYEGWKQAVA

3D structure

• PDB: 1xup Structures of Enterococcal Glycerol Kinase in the Absence and Presence of Glycerol: Correlation of Conformation to Substrate Binding and a Mechanism of Activation by Phosphorylation
• Chain: O
• Resolution: 2.75 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.30: glycerol kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GOL	O	R84 E85 W104	R79 E80 W99

Gene Ontology

Molecular Function

• GO:0004370 glycerol kinase activity
• GO:0005524 ATP binding
• GO:0016301 kinase activity
• GO:0016773 phosphotransferase activity, alcohol group as acceptor

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0006071 glycerol metabolic process
• GO:0006072 glycerol-3-phosphate metabolic process
• GO:0016310 phosphorylation
• GO:0019563 glycerol catabolic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:1xup, PDBe:1xup, PDBj:1xup
• PDBsum: 1xup
• PubMed: 14717590
• UniProt: O34153|GLPK_ENTCA Glycerol kinase (Gene Name=glpK)