Structure of PDB 8fl0 Chain NC

Receptor sequence

>8fl0NC (length=432) Species: 9606 (Homo sapiens)

MRDRATIRRLNMYRQKERRNSRGKIIKPLQYQSTVASGTVARVEPNIKWF
GNTRVIKQSSLQKFQEEMKDPYKVVMKQSKLPMSLLHDRIRPHNLKVHIL
DTESFETTFGPKSQRKRPNLFASDMQSLIENAEMSTESYDQGKDRDLVTE
DTGVRNEAQEEIYKKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRSP
HIETYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASL
TNPFGKGAFIQLLRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKV
CNVAPIAGETKVWQYITLMRRIFLIDCPGVVYPSEDSETDIVLKGVVQVE
KIKSPEDHIGAVLERAKPEYISKTYKIDSWENAEDFLEKLAFRTGKLLKG
GEPDLQTVGKMVLNDWQRGRIPFFVKPPNAEP

3D structure

• PDB: 8fl0 Principles of human pre-60 S biogenesis.
• Chain: NC
• Resolution: 2.91 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	NC	M32 R33 R39 R50 N51 S52 S68 G69 P76 N77 I78 K79 T84 K112 H128 N129 L130 K131 H133 I134 L135 K147 Q149 K151 R180 R190 E196 K200 S203 R205 I206 W207 Y211 K309 Q310 K333 V335 G343 W348 Q349 Y350 T352 Y367 P368 V381 Q383 K386	M1 R2 R8 R19 N20 S21 S37 G38 P45 N46 I47 K48 T53 K77 H93 N94 L95 K96 H98 I99 L100 K112 Q114 K116 R145 R155 E161 K165 S168 R170 I171 W172 Y176 K274 Q275 K298 V300 G308 W313 Q314 Y315 T317 Y332 P333 V346 Q348 K351
BS02	GTP	NC	N255 K256 D258 H282 N320 G322 K323 S324 S325 V338 G343 E344 T345	N220 K221 D223 H247 N285 G287 K288 S289 S290 V303 G308 E309 T310
BS03	MG	NC	S324 T345	S289 T310

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003924 GTPase activity
• GO:0005525 GTP binding

Biological Process

• GO:0008150 biological_process
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0016020 membrane

External links

• PDB: RCSB:8fl0, PDBe:8fl0, PDBj:8fl0
• PDBsum: 8fl0
• PubMed: 37410842
• UniProt: Q13823|NOG2_HUMAN Nucleolar GTP-binding protein 2 (Gene Name=GNL2)