Structure of PDB 5t5i Chain N

Receptor sequence

>5t5iN (length=329) Species: 145261 (Methanothermobacter wolfeii)

GKNITVERTGEENRRLIFQDCLCAVCGLCGEICPVSAIEVNPTGAMVRTE
QEKSKIAIDENKCVLCGMCSSICPFQALDLQIDGTSIKELAEYPKIIKSA
EIDDETCIQCKACETACPQDAITITRELPERKDLVTGEIEIDKDTCIYCG
MCEEMCPVDAIEIDHQTPSSASPVVATDIRVDEDKCVHCGICKRICPVDA
IMQVCPEVTGTSYIDPELCVNCGWCQEICPVDAATVTKPFEGELIIDQDT
CQACETCVMVCPCNVLSFPKPEKPGEKTTKLHKDERFCIYCGACERSCPV
TAITVKRNRINTTPIRSKAWKNAFDSLLK

3D structure

• PDB: 5t5i The methanogenic CO2 reducing-and-fixing enzyme is bifunctional and contains 46 [4Fe-4S] clusters.
• Chain: N
• Resolution: 1.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	N	C281 C283 V285 C308 I309 Y310 C311 G312 C314	C261 C263 V265 C288 I289 Y290 C291 G292 C294
BS02	SF4	N	C271 Q272 A273 C274 E275 C277 L301 C318	C251 Q252 A253 C254 E255 C257 L281 C298
BS03	SF4	N	C125 I130 C239 V240 N241 C242 G243 C245	C117 I122 C219 V220 N221 C222 G223 C225
BS04	SF4	N	I110 C115 I116 Q117 C118 K119 C121 I132 C249 P250	I102 C107 I108 Q109 C110 K111 C113 I124 C229 P230
BS05	SF4	N	C154 I155 Y156 C157 G158 C160 I187 C204 P205 I209	C146 I147 Y148 C149 G150 C152 I179 C196 P197 I201
BS06	SF4	N	I147 C164 P165 I169 C194 V195 H196 C197 G198 C200	I139 C156 P157 I161 C186 V187 H188 C189 G190 C192
BS07	SF4	N	C41 I46 C71 V72 L73 C74 G75 C77	C33 I38 C63 V64 L65 C66 G67 C69
BS08	SF4	N	C31 V33 C34 G35 C37 C81 F83 A85 L86	C23 V25 C26 G27 C29 C73 F75 A77 L78

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

External links

• PDB: RCSB:5t5i, PDBe:5t5i, PDBj:5t5i
• PDBsum: 5t5i
• PubMed: 27846502
• UniProt: O74028