Structure of PDB 5afi Chain N

Receptor sequence

>5afiN (length=120) Species: 562 (Escherichia coli)

MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEP
LITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKC
GFRAGDNAPMAYIELVDRSE

3D structure

• PDB: 5afi Structure of the E. coli ribosome-EF-Tu complex at <3 angstrom resolution by Cs-corrected cryo-EM.
• Chain: N
• Resolution: 2.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	N	M1 R2 H3 R4 K5 S6 R8 Q9 N11 R12 S14 S15 H16 R17 Q18 R22 M24 H31 I34 K35 T36 T37 P39 K40 K42 R46 E49 T53 V60 R63 R64 A68 R71 N73 E74 R90 A91 G92 G93 T95 R96 K99 R103 G105 D106 N107 A108	M1 R2 H3 R4 K5 S6 R8 Q9 N11 R12 S14 S15 H16 R17 Q18 R22 M24 H31 I34 K35 T36 T37 P39 K40 K42 R46 E49 T53 V60 R63 R64 A68 R71 N73 E74 R90 A91 G92 G93 T95 R96 K99 R103 G105 D106 N107 A108

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5afi, PDBe:5afi, PDBj:5afi
• PDBsum: 5afi
• PubMed: 25707802
• UniProt: P0AG44|RL17_ECOLI Large ribosomal subunit protein bL17 (Gene Name=rplQ)