Structure of PDB 5i4l Chain M0

Receptor sequence

>5i4lM0 (length=211) Species: 559292 (Saccharomyces cerevisiae S288C)

ARRPARCYRYQKNKPYPKSRYNRAVPDSKIRIYDLGKKKATVDEFPLCVH
LVSNELEQLSSEALEAARICANKYMTTVSGRDAFHLRVRVHPFHVLRINK
MQQGMRGAWGKPHGLAARVDIGQIIFSVRTKDSNKDVVVEGLRRARYKFP
GQQKIILSKKWGFTNLDRPEYLKKREAGEVKDDGAFVKFLSKKGSLENNI
REFPEYFAAQA

3D structure

• PDB: 5i4l Amicoumacin A induces cancer cell death by targeting the eukaryotic ribosome.
• Chain: M0
• Resolution: 3.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	M0	R3 R4 A6 R7 C8 K13 N14 K15 P16 Y22 Y34 D35 K39 K40 S61 E63 A64 A67 R90 H92 F94 R98 I99 G114 M115 R116 G117 W119 Y157 K158 P160 D193 G194 F196 K198	R2 R3 A5 R6 C7 K12 N13 K14 P15 Y21 Y33 D34 K38 K39 S60 E62 A63 A66 R89 H91 F93 R97 I98 G104 M105 R106 G107 W109 Y147 K148 P150 D183 G184 F186 K188
BS02	rna	M0	Y11 E56 L57 I131 K202 K203 G204 S205 L206 N209	Y10 E55 L56 I121 K192 K193 G194 S195 L196 N199
BS03	MG	M0	R156 Q163	R146 Q153

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006415 translational termination

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5i4l, PDBe:5i4l, PDBj:5i4l
• PDBsum: 5i4l
• PubMed: 27296282
• UniProt: P41805|RL10_YEAST Large ribosomal subunit protein uL16 (Gene Name=RPL10)