Structure of PDB 8kg6 Chain M

Receptor sequence
>8kg6M (length=813) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
YANSTWEVLQYKDSGEPGVLEVFVTINGKVQNITFHIPKTIYMKFKSQTM
PLQKIKNCLIEKSSASLPNTLPESVFLEEKENCTSIFNDENVLGVFEGTI
TPHQRAIMDLGASVTFRSKAMGALGKGIQQGFEMKDLSMAENERYLSGFS
MDIGYLLHFPTSIGYEFFSLFKSWGDTITINASSLGQIYKQMFEKKKGKI
ETYSYLVDIKEDINFEFVYFTDISKLYRRLSQETTKLKEERGLQFLLLLQ
SPFITKLLGTIRLLNQMPIVKLSLQPTLLKKLVNHVLSSGSWISHLIKLS
QYSNIPICNLRLDSMDYIIDVLYARKLKKENIVLWWNEKAPLPDHGGIQN
DFDLNTSWIMNDSEFPKINNSGVYDNVVLDVGVDNLTVNTILTSALFVHD
AFSNDALNVLRGMLKEWWDEALKENSTADLLVNSLASWVQNPNAKLFDGL
LRYHVHNLTKKALLQLVNEFSALGSTIVYADRNQILIKTNKYSPENCYAY
SQYMMKAVRTNPMFSYLDLNIKRYWDLLIWMDKFNFSGLACDYTAVSQWQ
LKKFLSPIYQPEFEDWMMIILDSMLKTKQSYLKLNSSVENSLNGFSHLFS
KPLMKRVKKLFKNQQEFILDPQYEADYVIPVLPGSHLNVKNPLLELVKSL
CHVMLLSKSTILEIRTLRKELLKIFELREFAKVAEFKDPSLSLVVPDFLC
EYCFFISDIDFCKAAPESIFSCVRCHKAFNQVLLQEHLIQKLRSDIESYL
IQDLRCSRCHKVKRDYMSAHCPCAGAWEGTLPRESIVQKLNVFKQVAKYY
GFDILLSCIADLT
3D structure
PDB8kg6 Synergism between CMG helicase and leading strand DNA polymerase at replication fork.
ChainM
Resolution3.07 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN M C2108 C2130 C2133 C700 C722 C725
BS02 ZN M C2164 C2167 C2179 C756 C759 C771
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003690 double-stranded DNA binding
GO:0003697 single-stranded DNA binding
GO:0003729 mRNA binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0008310 single-stranded DNA 3'-5' DNA exonuclease activity
GO:0016787 hydrolase activity
GO:0032183 SUMO binding
GO:0046872 metal ion binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0000278 mitotic cell cycle
GO:0006260 DNA replication
GO:0006261 DNA-templated DNA replication
GO:0006272 leading strand elongation
GO:0006281 DNA repair
GO:0006284 base-excision repair
GO:0006287 base-excision repair, gap-filling
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0006302 double-strand break repair
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0007064 mitotic sister chromatid cohesion
GO:0031573 mitotic intra-S DNA damage checkpoint signaling
GO:0033314 mitotic DNA replication checkpoint signaling
GO:0035822 gene conversion
GO:0042276 error-prone translesion synthesis
GO:0045004 DNA replication proofreading
Cellular Component
GO:0005634 nucleus
GO:0005657 replication fork
GO:0008622 epsilon DNA polymerase complex
GO:0043231 intracellular membrane-bounded organelle
GO:0043596 nuclear replication fork

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8kg6, PDBe:8kg6, PDBj:8kg6
PDBsum8kg6
PubMed37730685
UniProtP21951|DPOE_YEAST DNA polymerase epsilon catalytic subunit A (Gene Name=POL2)

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