Structure of PDB 8aow Chain M

Receptor sequence

>8aowM (length=451) Species: 371094 (Chikungunya virus strain S27-African prototype)

PVYVDIDADSAFLKALQRAYPMFEVEPRQVTPNDHANARAFSHLAIKLIE
QEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLAS
AAGKVLDRNISGKIGDLQAVMAVPDTETPTFCLHTDVSCRQRADVAIYQD
VYAVHAPTSLYHQAIKGVRLAYWVGFDTTPFMYNAMAGAYPSYSTNWADE
QVLKAKNIGLCSTDLTEGRRGKLSIMRGKKLEPCDRVLFSVGSTLYPESR
KLLKSWHLPSVFHLKGKLSFTCRCDTVVSCEGYVVKRITMSPGLYGKTTG
YAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICDQMTGILATEVTP
EDAQKLLVGLNQTNTMKNYMIPVVAQAFSKWAKECRKDMEDEKLLGVRER
TLTCCCLWAFKKQKTHTVYKRPDTQSIQKVQAEFDSFVVSGLSIPLRTRI
K

3D structure

• PDB: 8aow Structural basis and dynamics of Chikungunya alphavirus RNA capping by nsP1 capping pores.
• Chain: M
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.-
  2.7.7.-
  2.7.7.19: polynucleotide adenylyltransferase.
  2.7.7.48: RNA-directed RNA polymerase.
  3.1.3.84: ADP-ribose 1''-phosphate phosphatase.
  3.4.22.-
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.1.74: mRNA 5'-phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	M	H79 E129 C134 C141	H77 E127 C132 C139
BS02	SAH	M	G65 P83 R85 D89 R92 T137 D138 Q151	G63 P81 R83 D87 R90 T135 D136 Q149
BS03	MGP	M	H37 A40 R41 D152 Y154 F241 Y248 E250 Y285	H35 A38 R39 D150 Y152 F239 Y246 E248 Y283
BS04	MG	M	D36 H37 S245	D34 H35 S243

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0008174 mRNA methyltransferase activity

Biological Process

• GO:0006396 RNA processing
• GO:0016556 mRNA modification

External links

• PDB: RCSB:8aow, PDBe:8aow, PDBj:8aow
• PDBsum: 8aow
• PubMed: 36913573
• UniProt: Q8JUX6|POLN_CHIKS Polyprotein P1234