Structure of PDB 7eu1 Chain M

Receptor sequence

>7eu1M (length=1055) Species: 3702 (Arabidopsis thaliana)

TNRSTVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDF
ARVQFTTLEVKSRAQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRL
DDIVLTVGFPESDEKRFCALEKWDGVRCWILTEKRRVEFWVWESGDCYKI
EVRFEDIIETLSCCVNGDASEIDAFLLKLKYGPKVFKRVTRYRFCKEDFD
FMWIRTTDFSGSKSIGTSTCFCLEVHNGSTMLDIFSGLPYYREDTLSLTY
VDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNALVHAQKISLFAASDM
ELIKILRGMSLETALVILKKLHQQSSICYDPVFFVKTQMQSVVKKASAYK
RLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTFV
EEDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEF
LAFSASQLRGNSVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQL
FSASRQTLIVRAQDVEQIPDIEVTTDGADYCFSDGIGKISLAFAKQVAQK
CGLSHVPSAFQIRYGGYKGVIAVDRSSFRKLSLRDSMLKFDSNNRMLNVT
RWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVHLSMLGNMLEDRDAAL
NVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSELKSRC
RILVPKGRILIGCMDEMGILEYGQVYVRVTIGKVVVTKNPCLHPGDIRVL
DAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQFFVSWDEKII
PSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVH
ADRDPEKARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLER
FEKPTYISESVFGKLYRAVKSSLAQRGFESFIETAKAHRDMYGEKLTSLM
IYYGAANEEEILTGILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMG
WFEKSCEDEQQKKKLASAWYYVTYNPNHRDEKLTFLSFPWIVGDVLLDIK
AENAQ

3D structure

• PDB: 7eu1 Pol IV and RDR2: A two-RNA-polymerase machine that produces double-stranded RNA.
• Chain: M
• Resolution: 3.86 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	M	D830 D832 D834	D785 D787 D789

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003727 single-stranded RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0005515 protein binding
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding
• GO:0071667 DNA/RNA hybrid binding

Biological Process

• GO:0009751 response to salicylic acid
• GO:0010468 regulation of gene expression
• GO:0010495 siRNA-mediated long-distance post-transcriptional gene silencing
• GO:0030422 siRNA processing
• GO:0031047 regulatory ncRNA-mediated gene silencing
• GO:0050832 defense response to fungus
• GO:0140745 siRNA transcription

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus

External links

• PDB: RCSB:7eu1, PDBe:7eu1, PDBj:7eu1
• PDBsum: 7eu1
• PubMed: 34941388
• UniProt: O82504|RDR2_ARATH RNA-dependent RNA polymerase 2 (Gene Name=RDR2)