Structure of PDB 7e0l Chain M

Receptor sequence

>7e0lM (length=484) Species: 79929 (Methanothermobacter marburgensis str. Marburg)

KYRCKVCDYIYDPEVGDPTSGIKPGTPFQELPEDWLCPVCNVGKDQFEPL
PEDIDMFCYQCSQTVRGRACTVKGVCGKEATVARLQDNLLFAIKGISAYL
YHARELGYTDEVVDAFLERGFYSTLTNVNFDAEEFVSLALEAGEMNLRTM
KLLKKAHMDTYGEPEPAEVRVGALDGPAIIATGHSLKALEELLKQTEGSG
VNVYTHSELLPAHGYPGLRKYPHLAGQLGGPWFDQRETFSRYSAAVLGTS
NCVLLPRDSYRDRMFTCGVARLPGVEHVDGYDFSPVIEKALELPPLKEED
SATLTTGFGLSTILSLADKIKELVEEGKIRRFFLVGGCDSPLPQAKYYTE
FVRKLPEDTVVLTLACGKYRFNSMDLGDIDGIPRLIDLGQCNDSIVAVEL
VEALSNLFSMDVNELPLSIVLSWMEQKAAAILWSLLSLNLRGMYIGPILP
GWANDDIINVLVDKYELTPIGDPEEDIKKMMEVD

3D structure

• PDB: 7e0l Class III hybrid cluster protein homodimeric architecture shows evolutionary relationship with Ni, Fe-carbon monoxide dehydrogenases
• Chain: M
• Resolution: 2.82 Å

Enzymatic activity

• Enzyme Commision number: 1.7.99.1: hydroxylamine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FS2	M	H193 E217 W241 N260 C261 C347 C375 C400 M433 E434	H184 E208 W232 N251 C252 C338 C366 C391 M424 E425
BS02	SF4	M	C67 Y68 Q69 C70 T73 C79 G83 V84 C85	C58 Y59 Q60 C61 T64 C70 G74 V75 C76
BS03	FE	M	C5 C8 C38 C41	C4 C7 C37 C40

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004601 peroxidase activity
• GO:0005506 iron ion binding
• GO:0016491 oxidoreductase activity
• GO:0016661 oxidoreductase activity, acting on other nitrogenous compounds as donors
• GO:0046872 metal ion binding
• GO:0050418 hydroxylamine reductase activity
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0042542 response to hydrogen peroxide
• GO:0098869 cellular oxidant detoxification

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7e0l, PDBe:7e0l, PDBj:7e0l
• PDBsum: 7e0l
• UniProt: D9PYV4