Structure of PDB 7aod Chain M

Receptor sequence

>7aodM (length=1384) Species: 284812 (Schizosaccharomyces pombe 972h-)

MNIAQPVSSEIKSVKFGIYDVDDVEKISVKQIVNPVLLDNLNHPTNGGLY
DLALGPYLKNSVCATCHLDERYCPGHFGHIVLPIPAYHPLFFSQMYNLLR
STCLYCHHFKLSKVKVHLFFCRLKLLDYGLLNESEMVENVSLAKDAATLM
RIRDEFVAKSIADSRQLTTLLLHERKKVVRAFYHAISSRKQCDNCQSFSP
NFRKEGFAKIFEIPLSGKNLQFKYMTSTEVRNHLRRLFVKENVVLSRLYP
ASADMFFLQNIAVPPTRFRPNIQNELLTRILQSSIQIASLSKDSTVEERR
SRAFELLINAFVQLQHDVNSLIDSNRNPPPGIKQILEKKEGLFRKHMMGK
RVNYAARSVISPDPNIETNEIGVPPVFATKLTYPEPVTLYNFNEMRNAVI
NGPHKWPGASHIQNEDGTLISLMPLTIEQRTALANQLLTPQSNLISSPYS
YSRLINTNKKVYRHVRNGDMLILNRQPTLHKPSMMAHKARILPGEKTIRM
HYANCNSYNADFDGDEMNMHFPQSTNARSEAQFIANTDSQYLVPTSGDPL
RGLIQDHVVMGVWLTCKDTFYTRDEYQQLLFQALKPDETGMYGRIKTLPP
AIQRPGIYWTGKQIISSVLLNLKPSDRPGLNLKSKAKVPGKYWSPDSEEG
SVLFDDGELLCGILDKSSFGASAFGLVHSVHELYGPDIAGRLLSVLSRLF
TAYAQMRGFTCRMDDLRLDEQGDNWRRQLLENGKSFGLEAASEYVGLSTD
SPIALLNANLEEVYRDDEKLQGLDAAMKGKMNGLTSSIINKCIPDGLLTK
FPYNHMQTMTVSGAKGSNVNVSQISCLLGQQELEGRRVPLMVSGKSLPSF
VPYETSAKSGGFIASRFLTGIAPQEYYFHCMAGREGLIDTGYLQRCLMKH
LEGLCVQYDHTVRDSDGSIVQFHYGEDSLDVTKQKHLTQFEFSAKNYKSL
IQKYKVKSVLSAVDSETASSYAKKALKKPYKYDPVLDKYPPSRYLGSVSE
KFQRAVDEYTQKNPDKLIAESKLDDSLLNESKFKALMQLRYQQSLVDPGE
SVGVLASQSIGEPSTQMTLNTNVTLGIPRLREIIMTASANIQTPTMTLRL
NDGVSDKRASAFCKEVNKLVLSEVVRQVRVTEKTYAIRLDLYSRDEYQDE
YGVLQEEIESTFSNRFLKILNRIIKSYLAKSKQNCKHLDFDYVNGEWATV
ELVFPINTEKLLMVSLVEKACSETVIHEIPGITRCFSKPDTVPKVITEGV
NLKAIWEFYNEISMNDIYTNDIAAILRIYGVEAARNAIVHEVSSVFGVYG
IAVDPRHLSLIADYMTFEGGYKAFNRMGIEYNTSPFAKMSFETTCHFLTE
AALRGDVDDLSNPSSRLVVGRVGNFGTGSFDIFT

3D structure

• PDB: 7aod Conserved strategies of RNA polymerase I hibernation and activation.
• Chain: M
• Resolution: 4.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	M	C63 C66 H76	C63 C66 H76
BS02	ZN	M	C106 C228 C231	C106 C192 C195

Gene Ontology

Molecular Function

• GO:0001054 RNA polymerase I activity
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006362 transcription elongation by RNA polymerase I

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005736 RNA polymerase I complex
• GO:0005829 cytosol

External links

• PDB: RCSB:7aod, PDBe:7aod, PDBj:7aod
• PDBsum: 7aod
• PubMed: 33536435
• UniProt: P15398|RPA1_SCHPO DNA-directed RNA polymerase I subunit rpa1 (Gene Name=rpa1)