Structure of PDB 6gen Chain M

Receptor sequence

>6genM (length=688) Species: 559292 (Saccharomyces cerevisiae S288C)

SVVDVPVPSLLRGNLRTYQKQGLNWLASLYNNHTNGILADEMGLGKTIQT
ISLLAYLACEKENWGPHLIVVPTSVLLNWEMEFKRFAPGFKVLTYYGSPQ
QRKEKRKGWNKPDAFHVCIVSYQLVVQDQHSFKRKRWQYMVLDEAHNIKN
FRSTRWQALLNFNTQRRLLLTGTPLQNNLAELWSLLYFLMPQTVIDGKKV
SGFAETKKTVAKLHQVLRPYLLRRLKADVEKQMPAKYEHIVYCKLSKRQR
FLYDDFMSRAQTKATLASGNFMSIVNCLMQLRKVCNHPNLFEVRPILTSF
VLEHCVASDYKDVERTLLKLFKKNNQVNRVDLDFLNLVFTLNDKDLTSYH
AEEISKLTCVKNFVEEVNKLRETNKQLQEEFGEASFLNFQDANQYFKYSN
KQKLEGTVDMLNFLKMVNKLRCDRRPIFGKNLIDLLTKDRRVKYDKSSII
DNELIKPLQTRVLDNRKIIDTFAVLTPSAVSLDMRKLALGLNDDSSVGEN
TRLKVMQNCFEVSNPLHQLQTKLTIAFPDKSLLQYDCGKLQKLAILLQQL
KDNGHRALIFTQMTKVLDVLEQFLNYHGYLYMRLDGATKIEDRQILTERF
NTDSRITVFILSSRSGGLGINLTGADTVIFYDSDWNPAMDKQCQDRCHRI
GQTRDVHIYRFVSEHTIESNILKKANQKRQLDNVVIQE

3D structure

• PDB: 6gen Structure and dynamics of the yeast SWR1-nucleosome complex.
• Chain: M
• Resolution: 3.6 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	M	K814 R815 N831 T835 R836 M980	K133 R134 N150 T154 R155 M272
BS02	dna	M	T754 S779 R787 K788 N791 Q804 L805 Q808 H811 M980 N984 M1271 T1272 G1294 R1322 S1323	T73 S98 R106 K107 N110 Q123 L124 Q127 H130 M272 N276 M563 T564 G586 R614 S615
BS03	ADP	M	N695 Q700 G726 K727 T728 I729 N1329 R1357	N14 Q19 G45 K46 T47 I48 N621 R649
BS04	BEF	M	M723 K727 R1354 R1357	M42 K46 R646 R649

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004386 helicase activity
• GO:0005198 structural molecule activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0042393 histone binding
• GO:0060090 molecular adaptor activity
• GO:0140658 ATP-dependent chromatin remodeler activity

Biological Process

• GO:0000725 recombinational repair
• GO:0006325 chromatin organization
• GO:0006338 chromatin remodeling
• GO:0006355 regulation of DNA-templated transcription

Cellular Component

• GO:0000785 chromatin
• GO:0000812 Swr1 complex
• GO:0005634 nucleus
• GO:0005829 cytosol

External links

• PDB: RCSB:6gen, PDBe:6gen, PDBj:6gen
• PDBsum: 6gen
• PubMed: 30309918
• UniProt: Q05471|SWR1_YEAST Helicase SWR1 (Gene Name=SWR1)