Structure of PDB 6gan Chain M

Receptor sequence

>6ganM (length=551) Species: 83333 (Escherichia coli K-12)

SQRITIDPVTRIQGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPR
DAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTT
HDHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKV
QNKIKDLVASGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDA
NRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERLMYIKSFIDKLS
DFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPG
GYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIP
AYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEI
VAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIG
KGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVP
STWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAV
H

3D structure

• PDB: 6gan Mechanistic Exploitation of a Self-Repairing, Blocked Proton Transfer Pathway in an O2-Tolerant [NiFe]-Hydrogenase.
• Chain: M
• Resolution: 1.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q14 C61 C64 H68 R479 S502 C546 C549
• Catalytic site (residue number reindexed from 1): Q13 C60 C63 H67 R478 S501 C545 C548
• Enzyme Commision number: 1.12.99.6: hydrogenase (acceptor).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	F3S	M	K211 Q216	K210 Q215
BS02	SF4	M	R59 H214	R58 H213
BS03	FCO	M	C64 P478 R479 L482 V500 P501 S502 C546 C549	C63 P477 R478 L481 V499 P500 S501 C545 C548
BS04	MG	M	E42 A498 H552	E41 A497 H551

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0008901 ferredoxin hydrogenase activity
• GO:0016151 nickel cation binding
• GO:0016491 oxidoreductase activity
• GO:0033748 hydrogenase (acceptor) activity
• GO:0046872 metal ion binding

Cellular Component

• GO:0005886 plasma membrane

External links

• PDB: RCSB:6gan, PDBe:6gan, PDBj:6gan
• PDBsum: 6gan
• PubMed: 30070475
• UniProt: P0ACE0|MBHM_ECOLI Hydrogenase-2 large chain (Gene Name=hybC)