Structure of PDB 5wf0 Chain M

Receptor sequence

>5wf0M (length=136) Species: 562 (Escherichia coli)

MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAAR
RAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGK
VLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM

3D structure

• PDB: 5wf0 Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
• Chain: M
• Resolution: 3.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	M	R6 K8 F9 K11 H13 K14 G15 R16 R18 Q22 F28 R40 R44 Q45 A48 R55 K62 W64 F68 D70 E75 K76 L78 A79 V80 R81 M82 G83 K84 G85 K86 A122 K123 P125 K127	R6 K8 F9 K11 H13 K14 G15 R16 R18 Q22 F28 R40 R44 Q45 A48 R55 K62 W64 F68 D70 E75 K76 L78 A79 V80 R81 M82 G83 K84 G85 K86 A122 K123 P125 K127
BS02	rna	M	R16 R18 G19 R38	R16 R18 G19 R38

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5wf0, PDBe:5wf0, PDBj:5wf0
• PDBsum: 5wf0
• PubMed: 29733411
• UniProt: P0ADY7|RL16_ECOLI Large ribosomal subunit protein uL16 (Gene Name=rplP)