Structure of PDB 5guw Chain M

Receptor sequence

>5guwM (length=538) Species: 208964 (Pseudomonas aeruginosa PAO1)

AAEQYQGAASAVDPAHVVRTNGAPDMSESEFNEAKQIYFQRCAGCHGVLR
KGATGKPLTPDITQQRGQQYLEALITYGTPLGMPNWGSSGELSKEQITLM
AKYIQHTPPQPPEWGMPEMRESWKVLVKPEDRPKKQLNDLDLPNLFSVTL
RDAGQIALVDGDSKKIVKVIDTGYAVHISRMSASGRYLLVIGRDARIDMI
DLWAKEPTKVAEIKIGIEARSVESSKFKGYEDRYTIAGAYWPPQFAIMDG
ETLEPKQIVSTRGMTVDTQTYHPEPRVAAIIASHEHPEFIVNVKETGKVL
LVNYKDIDNLTVTSIGAAPFLHDGGWDSSHRYFMTAANNSNKVAVIDSKD
RRLSALVDVGKTPHPGRGANFVHPKYGPVWSTSHLGDGSISLIGTDPKNH
PQYAWKKVAELQGQGGGSLFIKTHPKSSHLYVDTTFNPDARISQSVAVFD
LKNLDAKYQVLPIAEWADLGEGAKRVVQPEYNKRGDEVWFSVWNGKNDSS
ALVVVDDKTLKLKAVVKDPRLITPTGKFNVYNTQHDVY

3D structure

• PDB: 5guw Dynamics of nitric oxide controlled by protein complex in bacterial system
• Chain: M
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 1.7.2.1: nitrite reductase (NO-forming).
  1.7.99.1: hydroxylamine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	M	R46 C47 C50 H51 T59 L63 R71 Y75 L79 I80 T84 L86 G87 M88 P89 W91	R41 C42 C45 H46 T54 L58 R66 Y70 L74 I75 T79 L81 G82 M83 P84 W86
BS02	DHE	M	R156 H182 I183 R185 R198 R225 S226 Y245 A283 A284 I285 H327 R372 F425 W498 F533	R151 H177 I178 R180 R193 R220 S221 Y240 A278 A279 I280 H322 R367 F420 W493 F528

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0020037 heme binding

External links

• PDB: RCSB:5guw, PDBe:5guw, PDBj:5guw
• PDBsum: 5guw
• PubMed: 28847930
• UniProt: P24474|NIRS_PSEAE Nitrite reductase (Gene Name=nirS)