Structure of PDB 5gad Chain M

Receptor sequence

>5gadM (length=144) Species: 562 (Escherichia coli)

MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGF
EGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANI
IGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

3D structure

• PDB: 5gad Structures of the E. coli translating ribosome with SRP and its receptor and with the translocon.
• Chain: M
• Resolution: 3.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	M	N4 E10 G11 S12 K13 K14 G16 K17 R18 L19 G20 R21 G22 G24 S25 G28 K29 T30 G32 R33 G34 H35 K36 G37 Q38 K39 R41 S42 G43 R47 E51 G52 G53 Q54 M55 Y58 R59 R60 P62 K63 F64 G65 F66 S68 R69 K70 A71 E76 R78 N99 Q104 K109 L112 R126 T128 G130	N4 E10 G11 S12 K13 K14 G16 K17 R18 L19 G20 R21 G22 G24 S25 G28 K29 T30 G32 R33 G34 H35 K36 G37 Q38 K39 R41 S42 G43 R47 E51 G52 G53 Q54 M55 Y58 R59 R60 P62 K63 F64 G65 F66 S68 R69 K70 A71 E76 R78 N99 Q104 K109 L112 R126 T128 G130

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5gad, PDBe:5gad, PDBj:5gad
• PDBsum: 5gad
• PubMed: 26804923
• UniProt: P02413|RL15_ECOLI Large ribosomal subunit protein uL15 (Gene Name=rplO)