Structure of PDB 4k4x Chain M

Receptor sequence

>4k4xM (length=463) Species: 12072 (Coxsackievirus B3)

GEIEFIESSKDAGFPVINTPSKTKLEPSVFHQVFEGNKEPAVLRSGDPRL
KANFEEAIFSKYIGNVNTHVDEYMLEAVDHYAGQLATLDISTEPMKLEDA
VYGTEGLEALDLTTSAGYPYVALGIKKRDILSKKTKDLTKLKECMDKYGL
NLPMVTYVKDELRSIEKVAKGKSRLIEASSLNDSVAMRQTFGNLYKTFHL
NPGVVTGSAVGCDPDLFWSKIPVMLDGHLIAFDYSGYDASLSPVWFACLK
MILEKLGYTHKETNYIDYLCNSHHLYRDKHYFVRGGMPSGCSGTSIFNSM
INNIIIRTLMLKVYKGIDLDQFRMIAYGDDVIASYPWPIDASLLAEAGKG
YGLIMTPADKGECFNEVTWTNVTFLKRYFRADEQYPFLVHPVMPMKDIHE
SIRWTKDPKNTQDHVRSLCLLAWHNGEHEYEEFIRKIRSVPVGRCLTLPA
FSTLRRKWLDSFG

3D structure

• PDB: 4k4x Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts.
• Chain: M
• Resolution: 2.37 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	M	N18 P20 K22 K24 E108 T114 S115 K127 Y157 K159 I176 A178 R188 H199 G211 C212 D213 G290 C291 S292 Y327 R416	N18 P20 K22 K24 E108 T114 S115 K127 Y157 K159 I176 A178 R188 H199 G211 C212 D213 G290 C291 S292 Y327 R416
BS02	rna	M	K133 Y327 D329 D330 L375 K376 S401 N410 D413 H414 S417	K133 Y327 D329 D330 L375 K376 S401 N410 D413 H414 S417

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:4k4x, PDBe:4k4x, PDBj:4k4x
• PDBsum: 4k4x
• PubMed: 23667424
• UniProt: Q5UEA2