Structure of PDB 4k4i Chain M

Receptor sequence

>4k4iM (length=329) Species: 9606 (Homo sapiens)

ATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQE
ACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKT
AQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQT
VQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLL
DSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSE
FACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPG
RVLPTPTEKDVFRLLGLPYREPAERDWLE

3D structure

• PDB: 4k4i Structural basis for the binding and incorporation of nucleotide analogs with L-stereochemistry by human DNA polymerase lambda.
• Chain: M
• Resolution: 2.25 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D179 D181 D242
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	M	W274 T371 E465 E466 N467 Y505 R517 K521	W26 T123 E217 E218 N219 Y257 R269 K273
BS02	dna	M	W342 G343 A344 G345 K347 T348	W94 G95 A96 G97 K99 T100
BS03	dna	M	Y267 W274 R275 Y279 G304 G306 R308 M309 K312	Y19 W26 R27 Y31 G56 G58 R60 M61 K64
BS04	1RY	M	R386 S417 G426 Y505 S509 A510 N513 R517	R138 S169 G178 Y257 S261 A262 N265 R269
BS05	CA	M	C300 I302 P303 I305	C52 I54 P55 I57
BS06	CA	M	S339 I341 W342 A344	S91 I93 W94 A96
BS07	CA	M	D427 D429	D179 D181
BS08	CA	M	D427 D429	D179 D181

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4k4i, PDBe:4k4i, PDBj:4k4i
• PDBsum: 4k4i
• PubMed: 25015085
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)