Structure of PDB 3olb Chain M

Receptor sequence

>3olbM (length=461) Species: 12080 (Poliovirus 1)

GEIQWMRPSKEVGYPIINAPSKTKLEPSAFHYVFEGVKEPAVLTKNDPRL
KTDFEEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDA
MYGTDGLEALDLSTSAGYPYVAMGKKKRDILNKQTRDTKEMQKLLDTYGI
NLPLVTYVKDELRSKTKVEQGKSRLIEASSLNDSVAMRMAFGNLYAAFHK
NPGVITGSAVGCDPDLFWSKIPVLMEEKLFAFDYTGYDASLSPAWFEALK
MVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGCSGTSIFNSMI
NNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDY
GLTMTPADKSATFETVTWENVTFLKRFFRADEKYPFLIHPVMPMKEIHES
IRWTKDPRNTQDHVRSLCLLAWHNGEEEYNKFLAKIRSVPIGRALDLPEY
STLYRRWLDSF

3D structure

• PDB: 3olb Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase.
• Chain: M
• Resolution: 2.41 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	M	P20 K24 E108 T114 S115 Y157 K159 I176 A178 R188 H199 G211 C212 D213 S288 G289 S291 D412 R415 L419 R456	P20 K24 E108 T114 S115 Y157 K159 I176 A178 R188 H199 G211 C212 D213 S288 G289 S291 D412 R415 L419 R456
BS02	rna	M	R128 K133 Y326 L374 K375 M392 S400 R408 N409 D412 H413 S416 L420	R128 K133 Y326 L374 K375 M392 S400 R408 N409 D412 H413 S416 L420
BS03	rna	M	I16 G124	I16 G124
BS04	ZN	M	H272 C281	H272 C281
BS05	DCT	M	K159 R163 R174	K159 R163 R174

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:3olb, PDBe:3olb, PDBj:3olb
• PDBsum: 3olb
• PubMed: 21148772
• UniProt: B3VQP5