Structure of PDB 3ol8 Chain M

Receptor sequence

>3ol8M (length=461) Species: 12080 (Poliovirus 1)

GEIQWMRPSKEVGYPIINAPSKTKLEPSAFHYVFEGVKEPAVLTKNDPRL
KTDFEEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDA
MYGTDGLEALDLSTSAGYPYVAMGKKKRDILNKQTRDTKEMQKLLDTYGI
NLPLVTYVKDELRSKTKVEQGKSRLIEASSLNDSVAMRMAFGNLYAAFHK
NPGVITGSAVGCDPDLFWSKIPVLMEEKLFAFDYTGYDASLSPAWFEALK
MVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGCSGTSIFNSMI
NNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDY
GLTMTPADKSATFETVTWENVTFLKRFFRADEKYPFLIHPVMPMKEIHES
IRWTKDPRNTQDHVRSLCLLAWHNGEEEYNKFLAKIRSVPIGRALDLPEY
STLYRRWLDSF

3D structure

• PDB: 3ol8 Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase.
• Chain: M
• Resolution: 2.75 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	M	P20 K24 L43 E108 T114 S115 Y157 K159 I176 E177 A178 R188 H199 G211 C212 D213 G289 C290 R415 L419	P20 K24 L43 E108 T114 S115 Y157 K159 I176 E177 A178 R188 H199 G211 C212 D213 G289 C290 R415 L419
BS02	rna	M	K133 D238 S288 Y326 D328 D329 L374 K375 S400 N409 H413 L420	K133 D238 S288 Y326 D328 D329 L374 K375 S400 N409 H413 L420
BS03	rna	M	N18 G124	N18 G124
BS04	ZN	M	H270 H272 C281	H270 H272 C281
BS05	MN	M	D233 Y234 D328	D233 Y234 D328
BS06	MN	M	D233 D328 D329	D233 D328 D329

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:3ol8, PDBe:3ol8, PDBj:3ol8
• PDBsum: 3ol8
• PubMed: 21148772
• UniProt: B3VQP5