Structure of PDB 3ol7 Chain M

Receptor sequence

>3ol7M (length=461) Species: 12080 (Poliovirus 1)

GEIQWMRPSKEVGYPIINAPSKTKLEPSAFHYVFEGVKEPAVLTKNDPRL
KTDFEEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDA
MYGTDGLEALDLSTSAGYPYVAMGKKKRDILNKQTRDTKEMQKLLDTYGI
NLPLVTYVKDELRSKTKVEQGKSRLIEASSLNDSVAMRMAFGNLYAAFHK
NPGVITGSAVGCDPDLFWSKIPVLMEEKLFAFDYTGYDASLSPAWFEALK
MVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGCSGTSIFNSMI
NNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDY
GLTMTPADKSATFETVTWENVTFLKRFFRADEKYPFLIHPVMPMKEIHES
IRWTKDPRNTQDHVRSLCLLAWHNGEEEYNKFLAKIRSVPIGRALDLPEY
STLYRRWLDSF

3D structure

• PDB: 3ol7 Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase.
• Chain: M
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	M	P20 K24 E108 T114 S115 K127 Y157 K159 I176 A178 R188 H199 G211 C212 D213 G289 L419	P20 K24 E108 T114 S115 K127 Y157 K159 I176 A178 R188 H199 G211 C212 D213 G289 L419
BS02	rna	M	K133 D238 S288 Y326 D328 L374 K375 S400 N409 H413 L420	K133 D238 S288 Y326 D328 L374 K375 S400 N409 H413 L420
BS03	rna	M	N18 G124	N18 G124
BS04	MG	M	Y234 D328	Y234 D328
BS05	POP	M	R163 K167 R174 G236	R163 K167 R174 G236
BS06	ZN	M	H270 H272 C281	H270 H272 C281

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:3ol7, PDBe:3ol7, PDBj:3ol7
• PDBsum: 3ol7
• PubMed: 21148772
• UniProt: P03300|POLG_POL1M Genome polyprotein