Structure of PDB 8k2a Chain LO

Receptor sequence

>8k2aLO (length=287) Species: 9606 (Homo sapiens)

ARALDLLRGLPRVSLANLKPNPGSKKPERRPRGRRRGRKCGRGHKGERQR
GTRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYKPLSLNRLQYLIDLGR
VDPSQPIDLTQLVNGRGVTIQPLKRDYGVQLVEEGADTFTAKVNIEVQLA
SELAIAAIEKNGGVVTTAFYDPRSLDIVCKPVPFFLRGQPIPKRMLPPEE
LVPYYTDAKNRGYLADPAKFPEARLELARKYGYILPDITKDELFKMLCTR
KDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS

3D structure

• PDB: 8k2a Structural basis for differential inhibition of eukaryotic ribosomes by tigecycline.
• Chain: LO
• Resolution: 2.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	LO	L24 A25 L27 P29 N30 G32 K34 K35 E37 R38 R39 P40 R41 G42 R44 R45 G46 R47 K48 C49 G50 R51 H53 K54 G55 E56 R57 Q58 R59 T61 R62 P63 R64 F67 E68 G69 G70 Q71 T72 Y75 I76 R77 P79 K80 H87 S88 R100 R109 T128 Q130 L132 K133 R196	L15 A16 L18 P20 N21 G23 K25 K26 E28 R29 R30 P31 R32 G33 R35 R36 G37 R38 K39 C40 G41 R42 H44 K45 G46 E47 R48 Q49 R50 T52 R53 P54 R55 F58 E59 G60 G61 Q62 T63 Y66 I67 R68 P70 K71 H78 S79 R91 R100 T119 Q121 L123 K124 R187

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0032543 mitochondrial translation
• GO:1990830 cellular response to leukemia inhibitory factor

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005762 mitochondrial large ribosomal subunit
• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8k2a, PDBe:8k2a, PDBj:8k2a
• PDBsum: 8k2a
• PubMed: 38942792
• UniProt: Q9P015|RM15_HUMAN Large ribosomal subunit protein uL15m (Gene Name=MRPL15)