Structure of PDB 6syo Chain LLL

Receptor sequence

>6syoLLL (length=551) Species: 83333 (Escherichia coli K-12)

SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPR
DAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTT
HDHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKV
QNKIKDLVASGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDA
NRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERLMYIKSFIDKLS
DFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPG
GYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIP
AYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEI
VAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIG
KGDHTTFVKPNIPATGEFKGVGFLEAPKGMLSHWMVIKDGIISNYQAVVP
STWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAV
H

3D structure

• PDB: 6syo Hydrogen activation by NiFe-hydrogenases - consolidating the role of the pendant arginine.
• Chain: LLL
• Resolution: 1.25 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E14 C61 C64 H68 K479 S502 C546 C549
• Catalytic site (residue number reindexed from 1): E13 C60 C63 H67 K478 S501 C545 C548
• Enzyme Commision number: 1.12.99.6: hydrogenase (acceptor).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	LLL	R59 H214	R58 H213
BS02	FCO	LLL	C64 H68 P478 K479 L482 V500 P501 S502 C546 C549	C63 H67 P477 K478 L481 V499 P500 S501 C545 C548
BS03	MG	LLL	E42 A498 H552	E41 A497 H551
BS04	OXY	LLL	V63 C64 K479 C546	V62 C63 K478 C545

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0008901 ferredoxin hydrogenase activity
• GO:0016151 nickel cation binding
• GO:0016491 oxidoreductase activity
• GO:0033748 hydrogenase (acceptor) activity
• GO:0046872 metal ion binding

Cellular Component

• GO:0005886 plasma membrane

External links

• PDB: RCSB:6syo, PDBe:6syo, PDBj:6syo
• PDBsum: 6syo
• UniProt: P0ACE0|MBHM_ECOLI Hydrogenase-2 large chain (Gene Name=hybC)