Structure of PDB 8y0u Chain LG

Receptor sequence

>8y0uLG (length=233) Species: 4932 (Saccharomyces cerevisiae)

NPLTHSTPKNFGIGQAVQPKRNLSRYVKWPEYVRVQRQKKILSIRLKVPP
TIAQFQYTLDRNTAAETFKLFNKYRPETAAEKKERLTKEAAAVAEGKSKQ
DASPKPYAVKYGLNHVVALIENKKAKLVLIANDVDPIELVVFLPALCKKM
GVPYAIVKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTIDANFA
DKYDEVKKHWGGGILGNKAQAKMDKRAKNSDSA

3D structure

• PDB: 8y0u dormant ribosome with STM1
• Chain: LG
• Resolution: 3.59 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	LG	H28 S29 K32 N33 G37 Q41 R44 S47 R48 Y49 V50 K51 Y55 R57 R60 R68 K105 K106 R108 S126 K128 P129 K133 Y134 G135 L136 N137 H138 A141 N145 K147 V157 P159 L162 K183 A184 Q192 K193 S195 G239 N240 K241 A242 K248	H5 S6 K9 N10 G14 Q18 R21 S24 R25 Y26 V27 K28 Y32 R34 R37 R45 K82 K83 R85 S103 K105 P106 K110 Y111 G112 L113 N114 H115 A118 N122 K124 V134 P136 L139 K160 A161 Q169 K170 S172 G216 N217 K218 A219 K225
BS02	rna	LG	Y55 V56 Q59 R60 K63 R84 K181 R185	Y32 V33 Q36 R37 K40 R61 K158 R162

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8y0u, PDBe:8y0u, PDBj:8y0u
• PDBsum: 8y0u
• PubMed: 38698775
• UniProt: P17076|RL8A_YEAST Large ribosomal subunit protein eL8A (Gene Name=RPL8A)