Structure of PDB 8rxh Chain LG

Receptor sequence

>8rxhLG (length=241) Species: 347515 (Leishmania major strain Friedlin)

VASHFAARPKNFGIGQDVPYARDLSRFMRWPTFVTMQRKKRVLQRRLKVP
PALNQFTKVLDRASRNEALKLIKKYAPETRKARRERLQKVAEEKKKDPKK
TVSTKAPLAVVTGLQEVTRAIEKKQARMVVIANNVDPVELVLWMPNLCRA
NKIPYAIVKDMARLGDAIGRKTATCVALTDVNAEDEATLKNLIRSVNARF
LSRSDVIRRQWGGLQLSLRSRAELRKKHARNAGVDAAAIIQ

3D structure

• PDB: 8rxh Structural and mechanistic insights into the function of Leishmania ribosome lacking a single pseudouridine modification.
• Chain: LG
• Resolution: 2.93 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	LG	R52 P54 T55 F56 R103 K104 R106 R107 T135 G136 L137 Q138 E139 R142 K146 D159 M184 A185 R186 R193 K194 T195 T197	R29 P31 T32 F33 R80 K81 R83 R84 T112 G113 L114 Q115 E116 R119 K123 D136 M161 A162 R163 R170 K171 T172 T174
BS02	rna	LG	A29 R31 P32 K33 N34 F35 G36 I37 Q39 Y43 R45 L47 R49 F50 R52 K62 R64 V65 R68 R69 D84 R85 R88 K182 R217 A221 R222 L224 S225 R226 V229 L237 L239 L241 R242 R244 A245 R248 K249 H251	A6 R8 P9 K10 N11 F12 G13 I14 Q16 Y20 R22 L24 R26 F27 R29 K39 R41 V42 R45 R46 D61 R62 R65 K159 R194 A198 R199 L201 S202 R203 V206 L214 L216 L218 R219 R221 A222 R225 K226 H228
BS03	rna	LG	F56 V57 Q60 R61 R64 R88 K182 D183 R186	F33 V34 Q37 R38 R41 R65 K159 D160 R163

Gene Ontology

Molecular Function

• GO:0003723 RNA binding

Biological Process

• GO:0000470 maturation of LSU-rRNA

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8rxh, PDBe:8rxh, PDBj:8rxh
• PDBsum: 8rxh
• PubMed: 38722744
• UniProt: Q4QIP1