Structure of PDB 8k82 Chain LG

Receptor sequence

>8k82LG (length=231) Species: 4932 (Saccharomyces cerevisiae)

NPLTHSTPKNFGIGQAVQPKRNLSRYVKWPEYVRVQRQKKILSIRLKVPP
TIAQFQYTLDRNTAAETFKLFNKYRPETAAEKKERLTKEAAAVAEGKSKQ
DASPKPYAVKYGLNHVVALIENKKAKLVLIANDVDPIELVVFLPALCKKM
GVPYAIVKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTIDANFA
DKYDEVKKHWGGGILGNKAQAKMDKRAKNSD

3D structure

• PDB: 8k82 Structural basis for differential inhibition of eukaryotic ribosomes by tigecycline.
• Chain: LG
• Resolution: 3.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	LG	T27 H28 S29 T30 K32 N33 F34 G35 I36 Q38 Q41 P42 R44 L46 S47 R48 Y49 K51 E54 Y55 R60 L65 R68 K70 E100 K105 R108 S126 K128 P129 K133 Y134 G135 L136 N137 H138 A141 N145 V157 D158 L162 K183 A184 Q192 K193 S195 N240 K241 A242	T4 H5 S6 T7 K9 N10 F11 G12 I13 Q15 Q18 P19 R21 L23 S24 R25 Y26 K28 E31 Y32 R37 L42 R45 K47 E77 K82 R85 S103 K105 P106 K110 Y111 G112 L113 N114 H115 A118 N122 V134 D135 L139 K160 A161 Q169 K170 S172 N217 K218 A219
BS02	rna	LG	Y55 V56 Q59 R60 K63 R84 K181	Y32 V33 Q36 R37 K40 R61 K158

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8k82, PDBe:8k82, PDBj:8k82
• PDBsum: 8k82
• PubMed: 38942792
• UniProt: P17076|RL8A_YEAST Large ribosomal subunit protein eL8A (Gene Name=RPL8A)