Structure of PDB 8k2c Chain LF

Receptor sequence

>8k2cLF (length=225) Species: 9606 (Homo sapiens) [Search protein sequence]

NFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMAR
MARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFV
KLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNA
LIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKK
TTHFVEGGDAGNREDQINRLIRRMN

3D structure

PDB

8k2c Structural basis for differential inhibition of eukaryotic ribosomes by tigecycline.

Chain

Resolution

2.4 Å

3D
structure

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Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	LF	F25 E27 K31 R32 R34 K35 F37 Q39 R43 R46 R47 K48 Y51 E52 H58 K59 Y61 Y65 R66 I69 R70 M71 R73 M74 R76 A78 R96 G97 I98 N99 G100 P103 K104 K107 L111 R113 Q116 I117 F118 K124 K127 A128 N131 M132 L147 K148 N151 K156 R157 K165 R166 A176 G180 K181 Y182 I185 E188 R200 K202 N206 K212 L213 S214 S215 P216 R217 G218 G219 M220 K221 K222 K223 R242 R245	F2 E4 K8 R9 R11 K12 F14 Q16 R20 R23 R24 K25 Y28 E29 H35 K36 Y38 Y42 R43 I46 R47 M48 R50 M51 R53 A55 R73 G74 I75 N76 G77 P80 K81 K84 L88 R90 Q93 I94 F95 K101 K104 A105 N108 M109 L124 K125 N128 K133 R134 K142 R143 A153 G157 K158 Y159 I162 E165 R177 K179 N183 K189 L190 S191 S192 P193 R194 G195 G196 M197 K198 K199 K200 R219 R222
BS02	rna	LF	E137 K222 T224 T225 H226 E229	E114 K199 T201 T202 H203 E206

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0003729	mRNA binding
GO:0003735	structural constituent of ribosome
GO:0005515	protein binding
GO:0042802	identical protein binding

	Biological Process
GO:0000463	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0002181	cytoplasmic translation
GO:0006364	rRNA processing
GO:0006412	translation
GO:0042273	ribosomal large subunit biogenesis

	Cellular Component
GO:0005634	nucleus
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0005925	focal adhesion
GO:0014069	postsynaptic density
GO:0016020	membrane
GO:0022625	cytosolic large ribosomal subunit
GO:0022626	cytosolic ribosome
GO:1990904	ribonucleoprotein complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8k2c, PDBe:8k2c, PDBj:8k2c
PDBsum	8k2c
PubMed	38942792
UniProt	P18124\|RL7_HUMAN Large ribosomal subunit protein uL30 (Gene Name=RPL7)

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