Structure of PDB 8bsj Chain LF

Receptor sequence

>8bsjLF (length=289) Species: 5741 (Giardia intestinalis)

WVKVQKTRSYFKRFQVQFRRRREGKTDYQARKALIIQDKNKYATPRYRFV
VRRTNKDIICQVTAAELTKDRVVCAAYSHELPRYGIPVGLTNYAAAYATG
LLCGRRLLEKLSVGIKENALAQLYPGLDKATGEEYHPDENENEEVHAFKA
FLDIGLARASTGARVFAAMKGAVDAGLNIPHSMKRFPGYSKDGFDSSALR
DRIMGKHVGDYMAELKAEDEETYRKRFSKYIAAKVEPLRLEAMYTKAHAA
IRANPQAVKAKFEIKKPVRQARLTLKERKERIDAKKLEA

3D structure

• PDB: 8bsj Insights into translocation mechanism and ribosome evolution from cryo-EM structures of translocation intermediates of Giardia intestinalis.
• Chain: LF
• Resolution: 6.49 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	LF	R10 F13 K14 F16 V18 F20 R21 R22 R24 K27 T28 Y30 R33 R50 R54 T56 N57 K58 Q63 L69 T70 K71 D72 R73 V74 Y79 N94 Y95 S162 T163 A165 R166 H209 V210 Y213 R228 F229 S230 K231 Y232 K261 F264 K267 K268 R271 Q272 A273 R274 L275 L277 R280 R283 I284	R8 F11 K12 F14 V16 F18 R19 R20 R22 K25 T26 Y28 R31 R48 R52 T54 N55 K56 Q61 L67 T68 K69 D70 R71 V72 Y77 N92 Y93 S160 T161 A163 R164 H207 V208 Y211 R226 F227 S228 K229 Y230 K259 F262 K265 K266 R269 Q270 A271 R272 L273 L275 R278 R281 I282
BS02	rna	LF	W3 Q7 Y12 F16 Q17 Q19 R23 G26 T28 D29 A35 L36 K43 Y44 T46 R48 L113 I117 L122 E146 V147 H148 F150 F153 I156 L158 F168 P182 H183 S184 R187	W1 Q5 Y10 F14 Q15 Q17 R21 G24 T26 D27 A33 L34 K41 Y42 T44 R46 L111 I115 L120 E144 V145 H146 F148 F151 I154 L156 F166 P180 H181 S182 R185

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8bsj, PDBe:8bsj, PDBj:8bsj
• PDBsum: 8bsj
• PubMed: 36912103
• UniProt: E2RU47