Structure of PDB 8rxh Chain LB

Receptor sequence

>8rxhLB (length=404) Species: 347515 (Leishmania major strain Friedlin)

SHCKFEHPRHGHLGFLPRKRSRQIRGRARAFPKDDATQKPHLTSFMVFKA
GMTHIVRDVDRPGSKVNKKEVVEPVTILEAPPMVIVGIVGYRQTPVGLKT
IGTVWAHHTSVEFRRRYYKNWKQSAQLAFSRQKQFANTKEGKVAEARTLN
AFAKKASVIRVIAHTQLRKLRNHRVGVKKAHVQEIQVNGGSVAAKIALAK
SLLEKEVRVDSVFQQSEACDVCSVTKGHGTEGVVKRWGVACLPRKTHRGL
RKVACIGAWHPARVMYTVARAGQHGYHHRTQLNKKIYQIGRSVAVEPNQA
TTTYDLTAKTITPMGGFVGYGTVRNDYVMLKGSVSGPRRRVMTLRRPMAP
QTSRQLKEKIVLKFIDTSSKIGHGRFQTKKEKNQWFGPLKKDRIRREERL
RKER

3D structure

• PDB: 8rxh Structural and mechanistic insights into the function of Leishmania ribosome lacking a single pseudouridine modification.
• Chain: LB
• Resolution: 2.93 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	LB	R245 K246 H248	R244 K245 H247
BS02	rna	LB	S2 H3 K5 F6 E7 H8 P9 R10 H11 P18 R19 K20 R21 P83 R93 P96 V97 H109 K134 N138 T139 K140 R148 N151 R169 K170 E207 R209 T231 G233 K236 R237 A241 C242 R245 K246 T247 H248 R249 G250 L251 R252 K253 A255 C256 I257 G258 A259 W260 H261 A263 R264 V265 T268 R271 A272 Q274 N326 D327	S1 H2 K4 F5 E6 H7 P8 R9 H10 P17 R18 K19 R20 P82 R92 P95 V96 H108 K133 N137 T138 K139 R147 N150 R168 K169 E206 R208 T230 G232 K235 R236 A240 C241 R244 K245 T246 H247 R248 G249 L250 R251 K252 A254 C255 I256 G257 A258 W259 H260 A262 R263 V264 T267 R270 A271 Q273 N325 D326
BS03	rna	LB	P9 G12 H13 L14 G15 F16 R19 R26 R30 A31 K50 M53 T54 H55 P75 Y92 L99 T101 G103 T104 W106 Y118 L128 A129 F130 R132 Q133 R161 E185 T226 K227 Y267 H279 R280 T281 L283 N284 K332 S334 V335 S336 G337 M349 P351 R355 K371 I372 G373	P8 G11 H12 L13 G14 F15 R18 R25 R29 A30 K49 M52 T53 H54 P74 Y91 L98 T100 G102 T103 W105 Y117 L127 A128 F129 R131 Q132 R160 E184 T225 K226 Y266 H278 R279 T280 L282 N283 K331 S333 V334 S335 G336 M348 P350 R354 K370 I371 G372
BS04	rna	LB	R21 I25 R116 R117 K120 N121 K123 Q124 A126 R172 H174 R175 V176 G177 V178 K179 K180 K227 G228 G230 T231 Q274 Y277 V294 P298 T311 M315 G316 V319 G320 G322 T323 R325 R340 R341 S370 I372 G373 K380 K383 W386 F387 L390 K391 K392 R394	R20 I24 R115 R116 K119 N120 K122 Q123 A125 R171 H173 R174 V175 G176 V177 K178 K179 K226 G227 G229 T230 Q273 Y276 V293 P297 T310 M314 G315 V318 G319 G321 T322 R324 R339 R340 S369 I371 G372 K379 K382 W385 F386 L389 K390 K391 R393

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8rxh, PDBe:8rxh, PDBj:8rxh
• PDBsum: 8rxh
• PubMed: 38722744
• UniProt: Q4Q2Q6