Structure of PDB 8to1 Chain L

Receptor sequence
>8to1L (length=551) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
SQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVM
EEAPAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTR
EGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLIT
GFVDIDPELAREKFAELRAQYVVTRDTIKHATAQEEILKLSEVFKQFRLV
PKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETS
DTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQVKDIN
RRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGL
MKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK
LNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIG
DDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMR
FGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLD
D
3D structure
PDB8to1 Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy.
ChainL
Resolution2.8 Å
3D
structure
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Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna L Y425 K426 T429 W433 R441 R451 P453 H455 V582 R586 Y363 K364 T367 W371 R379 R389 P391 H393 V520 R524
BS02 dna L Q437 L573 E585 R588 Q375 L511 E523 R526
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0005515 protein binding
GO:0016987 sigma factor activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006355 regulation of DNA-templated transcription
GO:0009408 response to heat
GO:0010468 regulation of gene expression
GO:0045892 negative regulation of DNA-templated transcription
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1903865 sigma factor antagonist complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8to1, PDBe:8to1, PDBj:8to1
PDBsum8to1
PubMed38951624
UniProtP00579|RPOD_ECOLI RNA polymerase sigma factor RpoD (Gene Name=rpoD)

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