Structure of PDB 7oea Chain L

Receptor sequence

>7oeaL (length=1432)

EDDMACVKDLVSKYLADNERLSRQKLAFLVQTEPRMLLMEGLKLLSLCIE
IDSCNANGCEHNSEDKSVERILHDHGILTPSLCFVVPDGYKLTGNVLILL
ECFVRSSPANFEQKYIEDFKKLEQLKEDLKSVDINLIPLIDGRTSFYNEQ
IPDWVNDKLRDTLFSLLRYAQESNSLFEESEYSRLCESLSMSGVESLNVL
LDNRSSHYEEIIASCHQGINNKLTAHEVKLQIEEEYQVFRNRLRKGEITG
QFLKVDKSRLLNDFNNLYVDEVTATKDNIEHLIYQFKRASPILRFLYANI
GEKECQMQYWRSFLNKVKSLRILTRRKLLLIFDALILLASIHDQTRHKCS
KGWLGSCFISVNDRLVSLESTKRDLEKWVGRRQQSERSNTIQPPDKNQIL
ISMFQKTILKATAALKDVGISVEHYKINMEVICPDSYDLILNFDVSGVVP
TISYQRTKFPFIMGGVELLESTDLERLSSLSLALVNSMKTSSTVQVVRCK
EAYCQEFLLSGAEFQLIYQKTGECSKCYAINDNRVGEICSFYADPKRYFP
AIFSAEVLQTTVSTMISWVKDCSELEEQLCNINSLTKMILVLILAHPSKR
SQKLLQNLRYFIMAYVSDYHHKDLIDKLREELITDVEFLLYRLVRALVNL
ILSEDVKSMMTNRFKFILNISYMCHFITKETPDRLTDQIKCFEKFLEPKL
EFGHVSINPADVATEEELDDMVYNAKKFLSKEGCTSIKGPDYKKPGVSKR
FLSLLTSSFNNGSLFKESEVTTAMLKNLCFYSQESPQSYSSTGPDTGRLK
FSLSYKEQVGGNRELYIGDLRTKMFTRLIEDYFEALSLQLSGSCLNNERE
FENAILSMKLNVSLAHVSYSMDHSKWGPMMCPFLFLATLQNLIFQADIKG
RDYLSTLLTWHMHKMVEIPFNVVSAMMKSFIKAQLGTTQSITEDFFYSNF
QIGVVPSHVSSILDMGQGILHNTSDFYALISERFINYAISCICGGTIDAY
TSSDDQISLFDQVLTELMQRDPEEFKTLIEFHYYMSDQLNKFVSPKSVIG
RFVAEFKSRFYVWGDEVPLLTKFVAAALHNIKCKEPHQLAETIDTIIDQS
VANGVPVHLCNLIQKRTLSLLQYARYPIDPFLLNCETDVRDWVDGNRSYR
IMRQIERLIPDACGRIRSMLRKLYNKLKTGQLHEEFTTNYLSSEHLSSLS
NLCELLGVEPPSESDLEFSWLNLAAHHPLRMVPTIVKTIQNKLSSTFTRG
AQKLLSEAINKSAFQSSIASGFVGLCRTLGSKCVRGPNKESLYIKSIQSL
ISDIQGIEPLIDSHGVQYWRVPLNIRDGNEGVISYFRPLLWDYMCISLST
AIELGAWVLGEPKKVRVLEFFKHNPCDYFPLKPAAIKFLDLCVALDVNCE
ALSLVSHIVKKREEHYIVLSSELRLSHTRTHE

3D structure

• PDB: 7oea Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.
• Chain: L
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	L	S328 N331 K332 S335 R337 R342 D380 S499 L502 K509 T510 F585 S1446 Y1450 R1452 R1622 G1625 S1626 S1658	S312 N315 K316 S319 R321 R325 D363 S479 L482 K489 T490 F553 S1119 Y1123 R1125 R1277 G1280 S1281 S1313
BS02	ZN	L	H364 C366	H347 C349
BS03	MG	L	M1189 D1190 D1332	M871 D872 D1005

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039689 negative stranded viral RNA replication
• GO:0039694 viral RNA genome replication
• GO:0039696 RNA-templated viral transcription
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044423 virion component

External links

• PDB: RCSB:7oea, PDBe:7oea, PDBj:7oea
• PDBsum: 7oea
• PubMed: 34857749
• UniProt: A0A3S8NV63