Structure of PDB 6fpw Chain L

Receptor sequence
>6fpwL (length=581) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
STQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVITNAVSCGT
MFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPD
NANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQS
LSSWPKSSPGYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLM
GFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPCAINIDESGAVGA
VNMERLNLVQSIITRTADFINNVMIPDALAIGQFNKPWSEIGTGLSDKCV
LSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVDLVDPQQVQEFVDH
AWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAP
RWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLGR
ILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRG
VGFTEAPRGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAA
LMNTKMAIPEQPLEILRTLHSFDPCLACSTH
3D structure
PDB6fpw Mechanistic Exploitation of a Self-Repairing, Blocked Proton Transfer Pathway in an O2-Tolerant [NiFe]-Hydrogenase.
ChainL
Resolution1.35 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E28 C76 C79 H83 R509 T532 C576 C579
Catalytic site (residue number reindexed from 1) E27 C75 C78 H82 R508 T531 C575 C578
Enzyme Commision number 1.12.99.6: hydrogenase (acceptor).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 EJ2 L C76 C79 V82 H83 A507 P508 R509 V530 P531 T532 C576 C579 C75 C78 V81 H82 A506 P507 R508 V529 P530 T531 C575 C578
BS02 MG L E57 C528 H582 E56 C527 H581
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008901 ferredoxin hydrogenase activity
GO:0009055 electron transfer activity
GO:0016151 nickel cation binding
GO:0016491 oxidoreductase activity
GO:0033748 hydrogenase (acceptor) activity
GO:0046872 metal ion binding
Biological Process
GO:0006113 fermentation
GO:0009061 anaerobic respiration
GO:0009267 cellular response to starvation
GO:0019645 anaerobic electron transport chain
GO:1902421 hydrogen metabolic process
Cellular Component
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0030288 outer membrane-bounded periplasmic space
GO:0044569 [Ni-Fe] hydrogenase complex
GO:0098567 periplasmic side of plasma membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6fpw, PDBe:6fpw, PDBj:6fpw
PDBsum6fpw
PubMed30070475
UniProtP0ACD8|MBHL_ECOLI Hydrogenase-1 large chain (Gene Name=hyaB)

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