Structure of PDB 5zwm Chain L

Receptor sequence

>5zwmL (length=416) Species: 559292 (Saccharomyces cerevisiae S288C)

EKFNPFEILPESIELFRTLALISLSETAQILPKIVDLKRILQQQEIKLLP
FFNEIIPLIKSNIKLMHNFLISLYSRRFPELSSLIPSPLQYSKVISILEN
ENYSKNESDELFFHLENKAKLTREQILVLTMSMKTSFKNKEPLDIKTRTQ
ILEANSILENLWKLQEDIGQYIASKISIIAPNVCFLVGPEIAAQLIAHAG
GVLEFSRIPSCNIASIGKNKHLSHELHTLESGVRQEGYLFASDMIQKFPV
SVHKQMLRMLCAKVSLAARVDAGQKNGDRNTVLAHKWKAELSKKARKLSE
APSISETKALPIPEDQPKKKRAGRKFRKYKEKFRLSHVRQLQNRMEFGKQ
EQTVLDSYGEEVGLGMSNTSLQQAVGATSGSRRSAGNQAKLTKVMKHRIS
EANQQADEFLISLGHN

3D structure

• PDB: 5zwm Structures of the fully assembledSaccharomyces cerevisiaespliceosome before activation
• Chain: L
• Resolution: 3.4 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	L	K364 K366 R367 A368 G369 R370 K371	K318 K320 R321 A322 G323 R324 K325
BS02	rna	L	C257 N258 S261 K264 K266 H267 R280 Q281 R304 A308 K309 K340 K343 P348 S351 K354 K365 R367 F372 K374 Y375 K376 K378 F379 K436 T438 K439	C211 N212 S215 K218 K220 H221 R234 Q235 R258 A262 K263 K294 K297 P302 S305 K308 K319 R321 F326 K328 Y329 K330 K332 F333 K390 T392 K393

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0003723 RNA binding

Biological Process

• GO:0000244 spliceosomal tri-snRNP complex assembly
• GO:0000387 spliceosomal snRNP assembly
• GO:0000398 mRNA splicing, via spliceosome
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing

Cellular Component

• GO:0005634 nucleus
• GO:0005681 spliceosomal complex
• GO:0005687 U4 snRNP
• GO:0005739 mitochondrion
• GO:0046540 U4/U6 x U5 tri-snRNP complex
• GO:0071001 U4/U6 snRNP
• GO:0071011 precatalytic spliceosome
• GO:0097526 spliceosomal tri-snRNP complex

External links

• PDB: RCSB:5zwm, PDBe:5zwm, PDBj:5zwm
• PDBsum: 5zwm
• PubMed: 29794219
• UniProt: P49704|PRP31_YEAST Pre-mRNA-processing factor 31 (Gene Name=PRP31)