Structure of PDB 7eiz Chain K

Receptor sequence

>7eizK (length=523) Species: 2697049 (Severe acute respiratory syndrome coronavirus 2)

NVTGLFKDCSKVITGLHPTQAPTHLSVDTKFKTEGLCVDIPGIPKDMTYR
RLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATREAVG
TNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLIPL
MYKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFELTSMKYFVKIG
PERTCCLCDRRATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGN
LQSNHDLYCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWTIEYPIIGDE
LKINAACRKVQHMVVKAALLADKFPVLHDIGNPKAIKCVPQADVEWKFYD
AQPCSDKAYKIEELFYSYATHSDKFTDGVCLFWNCNVDRYPANSIVCRFD
TRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQLPFFYYSDSPC
ESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYNMMI
SAGFSLWVYKQFDTYNLWNTFTR

3D structure

• PDB: 7eiz Coupling of N7-methyltransferase and 3'-5' exoribonuclease with SARS-CoV-2 polymerase reveals mechanisms for capping and proofreading
• Chain: K
• Resolution: N/A

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	K	C210 C226 H229	C208 C224 H227
BS02	ZN	K	H257 H264 C279	H255 H262 C277
BS03	ZN	K	C452 C484 H487	C450 C482 H485

Gene Ontology

Molecular Function

• GO:0000175 3'-5'-RNA exonuclease activity
• GO:0004482 mRNA 5'-cap (guanine-N7-)-methyltransferase activity
• GO:0004532 RNA exonuclease activity
• GO:0008168 methyltransferase activity

External links

• PDB: RCSB:7eiz, PDBe:7eiz, PDBj:7eiz
• PDBsum: 7eiz
• PubMed: 34143953
• UniProt: P0DTD1|R1AB_SARS2 Replicase polyprotein 1ab (Gene Name=rep)