Structure of PDB 6s6x Chain K

Receptor sequence

>6s6xK (length=472) Species: 192 (Azospirillum brasilense)

QRMLGFVHTAQRMPDKRPAAERRQDFAEIYARFSDERANEQANRCSQCGV
PFCQVHCPVSNNIPDWLKLTSEGRLEEAYEVSQATNNFPEICGRICPQDR
LCEGNCVIEQSTHGAVTIGSVEKYINDTAWDQGWVKPRTPSRELGLSVGV
IGAGPAGLAAAEELRAKGYEVHVYDRYDRMGGLLVYGIPGFKLEKSVVER
RVKLLADAGVIYHPNFEVGRDASLPELRRKHVAVLVATGVYKARDIKAPG
SGLGNIVAALDYLTTSNKVSLGDTVEAYENGSLNAAGKHVVVLGGGDTAM
DCVRTAIRQGATSVKCLYRRDRKNMPGSQREVAHAEEEGVEFIWQAAPEG
FTGDTVVTGVRAVRIHLGVADATGRQTPQVIEGSEFTVQADLVIKALGFE
PEDLPNAFDEPELKVTRWGTLLVDHRTKMTNMDGVFAAGDIVRGASLVVW
AIRDGRDAAEGIHAYAKAKAEA

3D structure

• PDB: 6s6x Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.
• Chain: K
• Resolution: 3.5 Å

Enzymatic activity

• Enzyme Commision number: 1.4.1.13: glutamate synthase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	K	C48 S49 C51 C56 C109 V110	C45 S46 C48 C53 C106 V107
BS02	SF4	K	C60 N64 C99 C105 I121 E125	C57 N61 C96 C102 I118 E122
BS03	FAD	K	I98 G157 P158 A159 Y177 D178 Y180 L186 E220 V221 T241 Y244 D300 D304 D443 S449	I95 G154 P155 A156 Y174 D175 Y177 L183 E217 V218 T238 Y241 D297 D301 D440 S446

Gene Ontology

Molecular Function

• GO:0004355 glutamate synthase (NADPH) activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006537 glutamate biosynthetic process
• GO:0097054 L-glutamate biosynthetic process

External links

• PDB: RCSB:6s6x, PDBe:6s6x, PDBj:6s6x
• PDBsum: 6s6x
• PubMed: 31473159
• UniProt: Q05756|GLTD_AZOBR Glutamate synthase [NADPH] small chain (Gene Name=gltD)