Structure of PDB 6je4 Chain K

Receptor sequence

>6je4K (length=1060) Species: 604162 (Neisseria meningitidis 8013)

AAFKPNSINYILGLAIGIASVGWAMVEIDEEENPIRLIDLGVRVFERAEV
PKTGDSLAMARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENG
LIKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNETADK
ELGALLKGVAGNAHALQTGDFRTPAELALNKFEKESGHIRNQRSDYSHTF
SRKDLQAELILLFEKQKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQK
MLGHCTFEPAEPKAAKNTYTAERFIWLTKLNNLRILEQGSERPLTDTERA
TLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKDNAEASTLMEMKAY
HAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRLKDRI
QPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDNT
EEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRL
YEQQHGKCLYSGKEINLGRLNEKGYVEIDAALPFSRTWDDSFNNKVLVLG
SENQNKGNQTPYEYFNGKDNSREWQEFKARVETSRFPRSKKQRILLQKFD
EDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKRVFASNGQITNLLRG
FWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAHQKTHF
PQPWEFFAQEVMIRVFGKPDGKPEFEEADTLEKLRTLLAEKLSSRPEAVH
EYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKDL
EKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKA
VRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFAS
CHRGTGNINIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRP
CRLKKRPPVR

3D structure

• PDB: 6je4 Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States.
• Chain: K
• Resolution: 3.069 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	K	Y140 R144 G156 L158 L159 Y199 L246 M254 K266 N285 R287 K333 G334 K390 D418 K419 F420 R440 D442 R473 K656 F657 D658 Y672 R675 E845 T846 Q981 N1029 K1043 G1049 G1051 K1053 T1054	Y139 R143 G153 L155 L156 Y196 L243 M251 K263 N282 R284 K330 G331 K387 D415 K416 F417 R437 D439 R465 K648 F649 D650 Y664 R667 E823 T824 Q959 N1007 K1021 G1027 G1029 K1031 T1032
BS02	dna	K	P52 K53 N944 T946 M947 Y965 H1024 R1033	P51 K52 N922 T924 M925 Y943 H1002 R1011
BS03	rna	K	S57 L58 A59 R62 R63 R66 R69 R70 L71 R73 R74 H77 R78 R81 R83 R84 N108 P110 W111 H133 K136 R138 G139 Q143 G190 H191 I192 R193 N194 Q195 R196 R205 Q242 R243 A245 K269 F277 V421 Q422 Y441 P466 R473 R479 R485 K486 R493 R665 T670 R675 P834 R836 K837 M838 G840 Q841 G842 H843 V847 S849 K851 R861 N877 R880 E881 K909 K912 T917 Q918 Q919 K921 A922 V923 R924 R936 N939 W967 A970 K971 R1071 C1073 P1080 V1081 R1082	S56 L57 A58 R61 R62 R65 R68 R69 L70 R72 R73 H76 R77 R80 R82 R83 N107 P109 W110 H132 K135 R137 G138 Q142 G187 H188 I189 R190 N191 Q192 R193 R202 Q239 R240 A242 K266 F274 V418 Q419 Y438 P458 R465 R471 R477 K478 R485 R657 T662 R667 P812 R814 K815 M816 G818 Q819 G820 H821 V825 S827 K829 R839 N855 R858 E859 K887 K890 T895 Q896 Q897 K899 A900 V901 R902 R914 N917 W945 A948 K949 R1049 C1051 P1058 V1059 R1060

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:6je4, PDBe:6je4, PDBj:6je4
• PDBsum: 6je4
• PubMed: 31668930
• UniProt: C9X1G5|CAS9_NEIM8 CRISPR-associated endonuclease Cas9 (Gene Name=cas9)